Long-Term Treatment with Shengmai San-Derived Herbal Supplement (Wei Kang Su) Enhances Antioxidant Response in Various Tissues of Rats with Protection Against Carbon Tetrachloride Hepatotoxicity

다국어 초록 (Multilingual Abstract)

Wei Kang Su (WKS) is a commercial herbal product based on a Chinese herbal formula, Shengmai San. Here, we investigated the effects of long-term treatment with WKS on mitochondrial antioxidant status and functional ability, as well as heat shock protein (Hsp) 25/70 production, in various tissues of rats. WKS treatment enhanced mitochondrial antioxidant status and ATP generation capacity, as well as Hsp 25/70 production in various rat tissues. WKS treatment suppressed plasma reactive oxygen metabolite levels and protected against carbon tetrachloride hepatotoxicity in rats. Long-term WKS treatment may prevent diseases by enhancing the resistance of mitochondria to oxidative stress.

참고문헌 (Reference)

1 Verde V., "Use of N,N-dimethyl-p-phenylenediamine to evaluate the oxidative status of human plasma" 36 : 869-873, 2002

2 Tanguay RM, "Tissue-specific expression of heat shock proteins of the mouse in the absence of stress" 14 : 112-118, 1993

3 Chiu PY, "Time-dependent enhancement in mitochondrial glutathione status and ATP generation capacity by schisandrin B treatment decreases the susceptibility of rat hearts to ischemia-reperfusion injury" 19 : 43-51, 2003

4 Sastre J., "The role of mitochondrial oxidative stress in aging" 35 : 1-8, 2003

5 Recknagel RO, "The carbon tetrachloride hepatotoxicity model: free radicals and calcium homeostasis,In: Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. 3" CRC Press 3-16, 1992

6 McCord JM, "Superoxide dismutase, An enzymic function for erythrocuprein (hemocuprein)." 244 : 6049-6055, 1969

7 Liang SM, "Shengmai San?a renowned Traditional Chinese medicinal formula. In: Traditional Herbal Medicines for Modern Times?Shengmai San" Taylor & Francis 1-15, 2002

8 Yao HT, "Shengmai San reduces hepatic lipids and lipid peroxidation in rats fed on a high-cholesterol diet" 116 : 49-57, 2008

9 You JS, "Sheng-mai-san reduces adriamycin-induced cardiomyopathy in rats" 34 : 295-305, 2006

10 Wang N., "Sheng-Mai-San is protective against post-ischemic myocardial dysfunction in rats through its opening of the mitochondrial KATP channels" 66 : 763-768, 2002

11 Lee IY, "Sheng mai san, a Chinese herbal medicine, protects against renal ischaemic injury during heat stroke in the rat" 32 : 742-748, 2005

12 Chiu PY, "Schisandrin B enhances renal mitochondrial antioxidant status, functional and structural integrity, and protects against gentamicin-induced nephrotoxicity in rats" 31 : 602-605, 2008

13 Chen N., "Schisandrin B enhances cerebral mitochondrial antioxidant status and structural integrity, and protects against cerebral ischemia=reperfusion injury in rats" 31 : 1387-1391, 2008

14 Ko KM, "Schisandra Chinensis-derived antioxidant activities in Sheng Mai San a compound formulation, in vivo and in vitro" 9 : 203-206, 1995

15 Hayes JD, "Potential contribution of the glutathione S-transferase supergene family to resistance to oxidative stress" 22 : 193-207, 1995

16 Kakkar P., "Mitochondria: a hub of redox activities and cellular distress control" 305 : 235-253, 2007

17 Salvioli S., "Mitochondria, aging and longevity?a new perspective" 492 : 9-13, 2001

18 Jezek P., "Mitochondria in homeostasis of reactive oxygen species in cell, tissues, and organism" 37 : 2478-2503, 2005

19 Wolf CR, "Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its relationship to lipid peroxidation" 18 : 553-558, 1980

20 Preville X., "Mammalian small stress proteins protect against oxidative stress through their ability to increase glucose-6-phosphate dehydrogenase activity and by maintaining optimal cellular detoxifying machinery" 247 : 61-78, 1999

21 Ronner P., "Luminometric assays of ATP, phosphocreatine, and creatine for estimation of free ADP and free AMP" 275 : 208-216, 1999

22 Zhang GQ, "Long-term treatment with a Chinese herbal formula, Sheng-Mai-San, improves cardiac contractile function in aged rats: the role of Ca2þ homeostasis" 11 : 991-1000, 2008

23 Mates JM, "Intracellular redox status and oxidative stress: implications for cell proliferation, apoptosis, and carcinogenesis" 82 : 273-299, 2008

24 Zylicz M., "Insights into the function of Hsp70 chaperones" 51 : 283-287, 2001

25 Ichikawa H., "In vitro antioxidant potentials of traditional Chinese medicine, Shengmai San and their relation to in vivo protective effect on cerebral oxidative damage in rats" 25 : 898-903, 2002

26 Arrigo AP, "Hsp27: novel regulator of intracellular redox state" 52 : 303-307, 2001

27 Arrigo AP, "Hsp27 consolidates intracellular redox homeostasis by upholding glutathione in its reduced form and by decreasing iron intracellular levels" 7 : 414-422, 2005

28 Chiu PY, "Hepatoprotective mechanism of schisandrin B: role of mitochondrial glutathione antioxidant status and heat shock proteins" 35 : 368-380, 2003

29 Kregel KC, "Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance" 92 : 2177-2186, 2002

30 Guo S., "Heat shock protein 70 regulates cellular redox status by modulating glutathione-related enzyme activities" 12 : 245-254, 2007

31 Meister A., "Glutathione metabolism and its selective modification" 263 : 17205-17208, 1988

32 Zhu D., "Ginsenoside Rg1 protects rat cardiomyocyte from hypoxia=reoxygenation oxidative injury via antioxidant and intracellular calcium homeostasis" 108 : 117-124, 2009

33 Center for Drug Evaluation and Research, "Food and Drug Administration: Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers. Guidance for Industry, Pharmacology and Toxicology" Food and Drug Administration 2005

34 Ding XF, "Effects of shengmai for injection on cardiogenic shock" 32 : 2298-2305, 2007

35 Ip SP, "Effects of schisandrin B pretreatment on tumor necrosis factor-alpha induced apoptosis and Hsp70 expression in mouse liver" 6 : 44-48, 2001

36 Xue HL, "Effects of Shenmai Huoxue decoction on early diabetic peripheral neuropathy in rats" 3 : 31-34, 2005

37 van Haaften RI, "Effect of vitamin E on glutathione-dependent enzymes" 35 : 215-253, 2003

38 Geng QX, "Effect of combined therapy of shenmai and compound danshen injection on myocardial reperfusion injury after percutaneous coronary intervention in patients with acute myocardial infarction" 24 : 496-499, 2004

39 Sadrzadeh SM, "Effect of chronic ethanol feeding on plasma and liver alpha- and gamma-tocopherol levels in normal and vitamin E-deficient rats. Relationship to lipid peroxidation" 47 : 2005-2010, 1997

40 Zhang YC, "Effect of Shengmai injection on cardiac function and inflammatory reaction in patients with acute coronary syndrome" 14 : 107-110, 2008

41 Griffith OW, "Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine" 106 : 207-212, 1980

42 Mehlen P., "Constitutive expression of human hsp27, Drosophila hsp27, or human alpha B-crystallin confers resistance to TNF- and oxidative stress-induced cytotoxicity in stably transfected murine L929 fibroblasts" 154 : 363-374, 1995

43 Chiu PY, "Chronic schisandrin B treatment improves mitochondrial antioxidant status and tissue heat shock protein production in various tissues of young adult and middle-aged rats" 7 : 199-210, 2006

44 Wang NL, "Chinese herbal medicine, Shengmai San, is effective for improving circulatory shock and oxidative damage in the brain during heatstroke" 97 : 253-265, 2005

45 Burcham PC, "Carbonylscavenging drugs & protection against carbonyl stress-associated cell injury" 8 : 319-330, 2008

46 Ehrnsperger M., "Binding of nonnative protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation" 16 : 221-229, 1997

47 Kagan V., "Antioxidant effects of ubiquinones in microsomes and mitochondria are mediated by tocopherol recycling" 169 : 851-857, 1990

48 Xie JT, "Antioxidant effects of ginsenoside Re in cardiomyocytes" 532 : 201-207, 2006

49 Dorta DJ, "Antioxidant activity of flavonoids in isolated mitochondria" 22 : 1213-1218, 2008

50 Nishida H., "Amyloid-beta-induced cytotoxicity of PC-12 cell was attenuated by Shengmai-san through redox regulation and outgrowth induction" 104 : 73-81, 2007

51 Liu H., "Activation of heat shock factor by alkylating agents is triggered by glutathione depletion and oxidation of protein thiols" 271 : 4805-4812, 1996

52 Leung HY, "A Yang-invigorating Chinese herbal formula enhances mitochondrial functional ability and antioxidant capacity in various tissues of male and female rats" 8 : 238-247, 2005