The Protective Effect of Sodium Hyaluronate on the Cartilage of Rabbit Osteoarthritis by Inhibiting Peroxisome Proliferator-Activated Receptor-Gamma Messenger RNA Expression

다국어 초록 (Multilingual Abstract)

Purpose: The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-
HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-γ) in cartilage of rabbit
osteoarthritis (OA) model. Materials and Methods: Forty eight white rabbits were randomly divided into A, B,
and C groups. Group A was normal control group, B and C groups underwent unilateral anterior cruciate ligament
transection (ACLT). The rabbits in group B were injected normal saline after ACLT; and Group C received intraarticular1%
sodium hyaluronate (HA) injection 5 weeks after surgery, 0.3 mL once a week. At 11th week after
surgery, all the rabbits were sacrificed. The cartilage changes on the medial femoral condyles were graded
separately. Cartilage sections were stained with safranin-O and HE, and messenger RNA (mRNA) expression of
PPAR-γ was detected by using real time polymerase chain reaction (Real Time-PCR). Results: Cartilage
degeneration in group B was significantly more severe than in A and C injection group. The grey value of Safranin-
O of B group was higher than A and C groups. Expression of PPAR-γ mRNA in group B was higher than group A
and C. Conclusion: This study shows that Na-HA has a protective effect on articular cartilage degeneration, and
the inhibitory effect on the PPAR-γ mRNA expression may be one of therapeutic mechanism of Na-HA.

다국어 초록 (Multilingual Abstract)

Purpose: The purpose of this study was to study the protective effect and influence of sodium hyaluronate (Na-
HA) on mRNA expression of peroxisome proliferators-activated receptor gamma (PPAR-γ) in cartilage of rabbit
osteoarthritis (OA) model. Materials and Methods: Forty eight white rabbits were randomly divided into A, B,
and C groups. Group A was normal control group, B and C groups underwent unilateral anterior cruciate ligament
transection (ACLT). The rabbits in group B were injected normal saline after ACLT; and Group C received intraarticular1%
sodium hyaluronate (HA) injection 5 weeks after surgery, 0.3 mL once a week. At 11th week after
surgery, all the rabbits were sacrificed. The cartilage changes on the medial femoral condyles were graded
separately. Cartilage sections were stained with safranin-O and HE, and messenger RNA (mRNA) expression of
PPAR-γ was detected by using real time polymerase chain reaction (Real Time-PCR). Results: Cartilage
degeneration in group B was significantly more severe than in A and C injection group. The grey value of Safranin-
O of B group was higher than A and C groups. Expression of PPAR-γ mRNA in group B was higher than group A
and C. Conclusion: This study shows that Na-HA has a protective effect on articular cartilage degeneration, and
the inhibitory effect on the PPAR-γ mRNA expression may be one of therapeutic mechanism of Na-HA.

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