N-Butyl-N-(4-hydroxybutyl) Nitrosamune 투여후 백서 방광조직에서 Ras 암유전자 및 EGFR의 발현양상에 관한 면역조직화학적 연구 = Immunohistochemical Study of Ras and Epidermal Growth Factor Receptor Expression in BBN-Induced Bladder Tumor of Rat

국문 초록 (Abstract)

BBN 투여에 의해 실험적으로 유발된 방광암의 암화과정에 있어서 ras 암유전자와 EGFR의 발현양상을 조사하여 방광암의 암화과정을 이해하는데 기초자료를 제공하고자 본 연구에 착수하였다.

다국어 초록 (Multilingual Abstract)

There is increasing evidence that genes are involved in normal cell growth and differentiation and genes that encode for growth factors are important in determining the development and biologic aggressiveness of the bladder tumor.
Sequential epithelial changes (normal, simple hyperplasia, nodular or papillary hyperplasia, papilloma and superficial transitional cell carcinoma-Ta, TIS) were observed in urinary bladder of rat in proportion to the duration of BBN(N -butyl-4-hydroxybutyl nitrosamine) administration.
This study was undertaken to understand the roles of ras oncogenes & EGFR (epidermcl growth factor receptor) in carcinogenesis of BBN induced bladder tumor.
Immunohistochemical staining for ras and EGFR was performed on nonaldehyde, Carnoy's solution - fixed tissues from BBN induced artificial bladder tumor of rat.
The results are as follows ;
1.The most frequently noted pathological change observed on hematoxyline and eosin stained sections was mild epithelial hyperplasia of the urinary bladder mucosa in early stage of BBN treated rats. With the time sequence, severe hyperplasia and papillary hyperplasia were seen. In late stage, papilloma and superficial transitional cell carcinoma were developed in some of them.
2.The expression of ras oncogene was weak in control group, but its immunoreavtivity was strong in experimental group and layers of positive cells were increased along with duration of carcinogen exposure.
3.In immunohistochemical staining for EGFR, weak positive cells were seen in control group. With progression of pathologic changes of bladder mucosa and prolongation of duration of BBN exposure, immunoreactivity of the cells and positive cell layers of the mucosa were increased.
In summary, ras oncogene and EGFR may play an important role in tumor development and progression in BBN induced bladder tumor of rat. But further studies are required for its full significances of oncogene expression.

목차 (Table of Contents)

• Ⅰ.서론
• Ⅱ.실험재료 및 연구방법
• 1.실험동물
• 2.BBN에 의한 방광암 유발
• 3.조직의처리
• 4.면역조직화학 염색
• 5.조직의 검색
• Ⅲ.결과
• 1.육안적 관찰
• 2.병리조직학적 변화소견
• 3.Ras 암유전자 산물의 발현양상
• 4.EGFR의 발현양상
• Ⅳ.고찰
• Ⅴ.결론