한국 재래 산양의 전염성 농피성 피부병에서 orf virus의 검출과 B2L 유전자를 통한 계통발생학적 분석 = Molecular Detection and Characterization of Orf Virus from Outbreak of Contagious Pustular Dermatitis in Korean Indigenous Goats

다국어 초록 (Multilingual Abstract)

Orf virus (ORFV), a member of genus Parapoxvirus (family-Poxviridae), a causative agent of contagious
ecthyma in sheep and goat leading to a condition commonly known as vesicular dermatitis. Recently, twelve goats from
Iksan in Jeonbuk province were observed with clinical signs like necrotic vesicular lesions around the mucosa of mouth,
nasal cavity, eye, ear, teats, abdomen and groin. Based on these clinical symptoms, contagious ecthyma infection was
suspected. The skin scrapping was collected from lesions for isolation of DNA and subsequent PCR amplification of
ORFV specific 235 bp region of B2L gene. All of the samples were found positive by PCR analysis. Sequencing and
further phylogenetic analysis of the PCR product revealed 100% identity to Japan isolate of ORFV (Okinawa, GenBank
accession number AB080769), and showed 99.6% of similarity to New Zealand strain (NZ-2, GenBank accession number
U06671). It was concluded that ORFV strain detected in the present study is homologous to Japan isolate and New
Zealand strain. The PCR test based on amplification of B2L gene is a highly useful tools for rapid and specific diagnosis
of contagious ecthyma.

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