혈장단백질이 돼지동결정자의 생존성 , 두모형태 및 수태에 미치는 영향 = Effect of Plasma Proteins on Sperm Viability , Acrosome Morphology and Conception of Frozen - Thawed Boar Semen

다국어 초록 (Multilingual Abstract)

This experiment was carried out to investigate the effects of plasma proteins on the sperm survival index, acrosome morphology and fertilizing capacity of frozen-thawed boar semen. Diluted semen with modified Beltsville F_5(mBF5) containing 0.05% of Porcine Albumin (PA) was frozen into pellets of 0.10 ㎖ on Dry Ice. The pellets were transferred to liquid nitrogen for storage. For each insemination 10 ㎖ of pellets were removed from liquid nitrogen. The pellets were dumped into 100-㎖ beaker containing 45 ㎖ of modified Beltsville Thawing Solution (mBTS) at 37℃ and thawed. Each sow was inseminated twice with 8×10^9 spermatozoa per dose. The results obtained from this studies were as follows: 1. In the diluted semen with mBF_5 and BF_5 extender, the addition level of 0.05 and 0.20 percent of plasma proteins did not show any difference in the sperm survival index after first and second dilution. 2. Sperm survival index of frozen thawed semen containing 0.05% of PA was significantly higher (p$lt;0.01) than addition of any other plasma proteins in BTS and mBTS. In addition of 0.20% level of PA, sperm survival index of frozen thawed semen was significantly higher (P$lt;0.05) than any other treatments in mBTS, but was not show significant differences in BTS. 3. The percentage of normal acrosome was 88 to 94% in fresh semen of boars, but there were no significant differences among the Yorkshire, Hampshire, Duroc and Landrace. 4. In BTS, the percentage of normal acrosome of frozen thawed semen containing 0.05% or PA was significantly higher (P$lt;0.05) than any other treatments, but in addition of 0.20% level of plasma proteins did not show significant differences. 5. In mBTS, the percentages of normal acrosome of frozen thawed semen containing 0.05% of PA and BSA were significantly higher (P$lt;0.01) than any other treatments, also addition of 0.20% of PA and BSA were significantly (P$lt;0.05) different. And addition of 0.05% level of EA was significantly higher (P$lt;0.05) than PG, BG and C. 6. The percentage of normal acrosome of frozen semen thawed in mBTS was show significantly higher (P$lt;0.05) than in B`TS. 7. Pregnancy with semen frozen in mBFs containing 0.05% of pA and thawed in mBTS was 6/12 (50%) and the average litter size was 7.8 pigs.