규칙적인 트레드밀 운동이 사회적 고립에 따른 성인 흰쥐 해마의 BDNF, NGF 수준 및 봉선핵의 serotonin-, TPH-positive cells에 미치는 영향

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the effects of regular treadmill exericse after social isolation on brain-derived neurotrophic factor(BDNF), nerve growth factor(NGF) and 5-bromo-2'- deoxyuridine- 5'-mono-phosphate(BrdU) -positive cell in the hippocampus, and serotonin-, TPH-positive cells in the raphe nuclei, and depression behaviors in the adult rats. Forty male Sprague-Dawley rats (31 weeks) were divided into four groups: (1) group housing and control group (GCG, n = 10); (2) group housing and exercise group (GEG, n = 10); (3) isolated housing and control group (ICG, n = 10); and (4) isolated housing and exercise group (IEG, n = 10). After 2 week of housing under the normal condition of 3 animals per cage, rats were socially isolated via transfer to individual cages for 8 weeks. Rats were then subjected to treadmill exercise for 5 days per week for 8 weeks, during which time the speed of the treadmill was gradually increased. The blood corticosterone level in the ICG was intend to increase, whereas in the IEG was intend to decrease compared to the ICG. Compared to the GCG, levels of BDNF and NGF were significantly decreased in the ICG, and significantly increased in the IEG (p < .001 respectively). There were significantly more BrdU-positive cells in the IEG compared to the GCG and ICG(p < .001). Serotonin-, TPH-positive cells in the GEG and IEG were significantly increased compared to the GCG and ICG(p < .01, p < .001). In the forced swim test, immobility time was significantly increased in the ICG, and significantly decreased in the IEG compared to the ICG(p < .001). These results show that regular treadmill exercise after social isolation not only increases the levels of BDNF and NGF to induce survival of cells in the hippocampus, but also improves depression-like behavior by increasing the number of serotonin, TPH cells expression in the raphe nuclei.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 연구방법
• 1. 실험대상
• 2. 실험절차
• 3. 혈중 corticosterone 분석
• 4. 조직채취
• 5. BDNF, NGF protein levels
• 6. Immunohistochemistry
• 7. Forced swim test
• 8. 자료처리
• Ⅲ. 결과
• 1. 혈중 corticosterone 농도 변화
• 2. BDNF 및 NGF 단백질 수준 변화
• 3. BrdU-positive cell 수의 변화
• 4. Serotonin- 및 TPH-positive cells 수의변화
• 5. 우울행동 변화
• Ⅳ. 논의
• Ⅴ. 결론
• 참고문헌
• ABSTRACT

참고문헌 (Reference)

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