Genus-Specific Distribution and Pathovar-Specific Variation of the Glycinecin R Gene Homologs in Xanthomonas genomes

다국어 초록 (Multilingual Abstract)

Xanthomonas axonopodis pv. glycines produces bacteriocins called glycinecin, and two glycinecin genes,
glyA and glyR, were reported previously. In this paper, we describe genomic distribution and variation of
the glyR gene revealed by extensive Southern hybridization analysis. In contrast to the glyA gene present
only in X. axonopodis pv. glycines, the glyR gene was found to be distributed widely in all the pathovars
of Xanthomas genus. It was also found that the glyR gene is a multigene family while the glyA is a single
copy gene. Moreover, the copy number and the variation of the glyR multigene are unique to each pathovar
of Xanthomonas. The uniqueness can be easily detected by the patterns resulted from Southern hybridization
using the genomic digests. Thus, we suggest the glyR gene can serve as a useful genus-specific and pathovarspecific
DNA marker for Xanthomonas. One of the glyR homologs was further isolated from X. axonopodis
pv. glycines, and analyzed to be functional with strong inhibitory activity against several members of
Xanthomonas.

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