Anti-melanogenesis effect of CA in B16F10 murine melanoma cells and UVB induced skin hyperpigmented mice model

다국어 초록 (Multilingual Abstract)

As a medicinal herb, CA contains lots of active components and possesses various pharmacological activities. Recently l CA has been frequently applied in cosmetics for skin whitening. However, there are still many issues to be figured out, like consistent effects and unknown mechanisms. This study aimed to demonstrate the anti-melanogenesis activity of hexane fraction of CA (HCA) on UVB-induced skin hyperpigmentation. C57BL/6j mice were irradiated by UVB (100 mJ/cm², 5 times/week) for 4 weeks. HCA was applied to the left ear after irradiation for 8 weeks, and the right ear was used as an inter control. According to the gray value and Mexameter melanin index, HCA reduced melanin in ear skin compared to all controls. HCA notably declined melanin generation in αMSH-triggered B16/F10 melanocyte in a concentration-dependent manner. HCA treatment also markedly diminished the tyrosinase activity, gene expression, and protein level in B16/F10. Cellular cAMP and MITF were noticeably down-regulated by HCA. Consequently, we suggested that CA exerts an effect of anti-melanogenesis via reduction of tyrosinase that might be mainly mediated by regulating cAMP cascade and MITF pathway.