Lipopolysaccharide로 활성화된 Raw 264.7 세포에서 녹차세포수의 항염증 효과 = Anti-inflammatory Effect of Green Tea Cell Water in Activated Raw 264.7 Cells with LipopolysaccharideLipopolysaccharide로 활성화된 Raw 264.7 세포에서 녹차세포수의 항염증 효과

다국어 초록 (Multilingual Abstract)

Background: The biochemical properties of green tea extracts can generally be divided into 4 aspects: antioxidative, anticarcinogenic, anti-inflammatory and antiradiation activities. Green tea extracts have 20-fold more antioxidative activity than vitamin C and also have a wide range of anti-inflammatory activity. Therefore, it is presumed that it would play a role in the treatment of chronic inflammatory disorders. Pathophysiology of immunologic disorders involves overexpression of proinflammatory mediators, including nitric oxide (NO) and prostaglandin E2 (PGE2). Methods: After the treatment of different concentrations for Green Tea Cell Water in lipopolysaccharide (LPS)-treated Raw 264.7 cells, the levels of NO and PGE2 were measured in the media. Furthermore, we analyzed the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), and phosphorylation of inhibitory kappa Bα using western blot. Results: Green Tea Cell Water suppressed the LPS-induced expression of iNOS and COX-2 in a concentration-dependent manner. Phosphorylation of the inhibitory-κB was also inhibited. Conclusion: Our results suggest that Green Tea Cell Water may be a significant inflammatory factor and can be used a therapeutic modality in managing chronic inflammatory diseases. (Korean J Asthma Allergy Clin Immunol 2012; 32:115-121)