위릉채 추출물 및 생물전환 분획물의 만성호흡기 질환 효과 검증 = Verification of the effect of Potentillae Chinensis Chinensis Herba extract and bioconversion fraction on chronic respiratory diseases

다국어 초록 (Multilingual Abstract)

Evaluating the antioxidant efficacy using Potentillae Chinensis Herba extract, the anti-inflammatory efficacy was tested in respiratory mucosal epithelium, RAW264.7 cells, and zebrafish. As a result, antioxidant activity increased in a concentration-dependent manner in DPPH free radical scavenging and ABTS+ cation radical activities. As a result of MTT assay for cell experiments, the survival rate of NCI-H292 cells was reduced to less than 70% when treated at each concentration of 100 μg/ml, subsequent experiments were conducted at 50 μg/ml.
Anti-inflammatory efficacy evaluation, NO production, TNF-α, IL-1β, and PGE2 decreased, and COX-2 also decreased significantly at 50 μg/ml. The mucin protein expression of Potentillae Chinensis Herba extract and bioconverted extract, it was observed that MUC5AC expression was significantly reduced. In the zebrafish toxicity evaluation, concentrations below 50 μg/ml did not show embryotoxicity and showed anti-inflammatory efficacy by reducing NO production due to LPS.
The above results are valid to be valuable for use as a functional material that suppresses inflammation by helping the expression of Potentillae Chinensis Herba's respiratory mucus proteins.