Rapid MALDI Biotyper-based Identification and Cluster Analysis of Streptococcus iniae

다국어 초록 (Multilingual Abstract)

Streptococcus iniae causes seve re mortalities among cultured marine species, especially in the olive flounder (Paralichthys olivaceus), whic h is econo mically important in Korea and Japan. Recently, there has been growing concern regarding the emergence of S. iniae as a zoonotic pathogen. Here, 89 S.
iniae isolates obtain ed from di seased olive flounder s collected from 2003 to 2008 in Jeju Island, South Korea, were charac- terized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The results were aligned both with the available Bruker Daltonics data- base and with a new set of S. iniae data e ntries de velop ed in our laboratory, and the results were compared. When we used the Bruker Daltonics database, the 89 isolates yielded either “no reliable identification” or were incorrectly iden- tified as Streptococcus pyogenes at the genus le vel. When w e used the new data entries from our laboratory, in contrast, all of the isolates were correctly identified as S. iniae at the genus (100%) and species (96.6%) levels. We performed pro- teomic analysis, divided the 89 isolates into cluster I (51.7%), cluster II (20.2%), and cluster III (28.1%), and then used the MALDI Biotyper software to identify specific masspeaks that enabled discrimination between clusters and between Stre p- tococcus species. Our results suggest that the use of MALDI TOF MS could outperform the conventional methods, prov- ing easier, faster, cheaper and more efficient in properly identifying S. iniae . This strategy could fa cilitate the epide- miological and taxonomical study of this important fish pathogen.

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