Paeonia suffruticosa Andr . 의 약배양에 관한 연구 = Studies on the Anther Culture of Paeonia suffruticosa Andr .

다국어 초록 (Multilingual Abstract)

The anthers of late uninucleate microspore or early binucleate microspore stage of Paeonia suffruticosa Andr. (economic tree) were cultured on the modified Mufashige and Skoog`s medium suppliment with Keinetin, 2,4-D, and NAA for inducing haploid plants. The results are as follows; 1. Callus were induced from both anther locule and anther wall, where that from anther locule was identified as haploid. 2. Among 2,000 anthers cultured, fourteen haploid callus were developed. These haploid callus were clearly identified to be originated from the microspore in anther locule. 3. Kiploid callus were induced only 0.5 percent from the callus of endothelium of anther wall, septium of two neighboring anther locule, parenchyma tissues of connectives and or anther locules. 4. In the anther locule of Paeonia suffruticosa Andr. cultured in medium, swollen microspores, polynucleate microspores, multicellurar pollen grains, or callus mass was frequently observed. And the haploids were seemed to be caused by the callus originated from the reduced microspore.