Pathogen-induced MdWRKY1 in ‘Qinguan’ Apple Enhances Disease Resistance

다국어 초록 (Multilingual Abstract)

The WRKY transcription factors have important functions in plant-defense signalling networks. We isolated MdWRKY1 from the Chinese ‘Qinguan’ apple, which is resistant to Alternaria blotch or leaf spot. The MdWRKY1protein was targeted to the nucleus and activated the expression of a reporter gene, consistent with the functioning of a transcription factor. When plants were infected with the pathogen Alternaria alternata f. sp. mali, MdWRKY1 was induced dramatically. Similarly, treatment with hormones SA and MeJA increased transcription significantly. Overexpression in tobacco also enhanced resistance to Phytophthora parasitica var. nicotianae Tucker. These results suggest that MdWRKY1 is a positive regulator of the defense response in higher plants.

참고문헌 (Reference)

1 Marchive C, "and characterization of a Vitis vinifera transcription factor, VvWRKY1, and its effect on responses to fungal pathogens in transgenic tobacco plants" 58 : 1999-2010, 2007

2 Ulker B, "WRKY transcription factors: from DNA binding towards biological function" 7 : 491-498, 2004

3 Singh K, "Transcription factors in plant defense and stress responses" 5 : 430-436, 2002

4 Journot-Catalino N, "The transcription factors WRKY11 and WRKY17 act as negative regulators of basal resistance in Arabidopsis thaliana" 18 : 3289-3302, 2006

5 Pandey SP, "The role of WRKY transcription factors in plant immunity" 150 : 1648-1655, 2009

6 Li J, "The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylatemediated signals in plant defense" 16 : 319-331, 2004

7 Ulker B, "The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways" 226 : 125-137, 2007

8 Eulgem T, "The WRKY superfamily of plant transcription factors" 5 : 199-206, 2000

9 Rotem J, "The Genus Alternaria: Biology, Epidemiology, and Pathogenicity" American Phytopathological Society Press 11-34, 1994

10 Zhou QY, "Soybean WRKY-type transcription factor genes, GmWRKY13, GmWRKY21, GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants" 6 : 486-503, 2008

11 Lai Z, "Roles of Arabidopsis WRKY3 and WRKY4 transcription factors in plant responses to pathogens" 8 (8): 2008

12 Qiu DY, "Rice gene network inferred from expression profiling of plants overexpressing OsWRKY13, a positive regulator of disease resistance" 1 : 538-551, 2008

13 Gasic K, "RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction" 22 : 437-437, 2004

14 Chen C, "Potentiation of developmentally regulated plant defense response by AtWRKY18, a pathogen-induced Arabidopsis transcription factor" 129 (129): 706-716, 2002

15 Xu XP, "Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, WRKY60 transcription factors" 18 : 1310-1326, 2006

16 Song Y, "Overexpression of the stress-induced OsWRKY8 improves osmotic stress tolerance in Arabidopsis" 54 : 4671-4678, 2009

17 Wang HH, "Overexpression of rice WRKY89 enhances ultraviolet B tolerance and disease resistance in rice plants" 65 : 799-815, 2007

18 Mzid R, "Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens" 131 : 434-444, 2007

19 Zhang JZ, "Overexpression analysis of plant transcription factors" 6 : 430-440, 2003

20 Liu D, "Osmotin overexpression in potato delays development of disease symptoms" 91 : 1888-1892, 1994

21 Kumar V, "Nucleotide sequence of an osmotin cDNA from the Nicotiana tabacum cv. white burley generated by the polymerase chain reaction" 18 : 621-662, 1992

22 Shen QH, "Nuclear activity of MLA immune receptors links isolate-specific and basal disease-resistance responses" 315 : 1098-1103, 2007

23 Eulgem T, "Networks of WRKY transcription factors in defense signaling" 10 : 366-271, 2007

24 Harimoto Y, "Multiple copies of AMT2 are prerequisite for the apple pathotype of Alternaria alternata to produce enough AM-toxin for expressing pathogenicity" 74 : 222-229, 2008

25 Chujo T, "Involvement of the elicitor- induced gene OsWRKY53 in the expression of defense-related genes in rice" 1769 : 497-505, 2007

26 Alexander D, "Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis related protein 1a" 90 : 7327-7331, 1993

27 CaiM, "Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13, a gene regulating rice disease resistance" 31 : 86-96, 2008

28 Sujeeth N, "Hydroxyproline-rich glycoproteins accumulate in pearl millet after seed treatment with elicitor of defence responses against Sclerospora graminicola" 74 : 230-237, 2010

29 Zheng ZY, "Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae" 7 (7): 2007

30 Bulajic A, "First report of Alternaria mali on apples in Yugoslavia" 80 (80): 1996

31 Chen WQ, "Expression prowle matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses" 14 : 559-574, 2002

32 Dong J, "Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response" 51 : 21-37, 2003

33 Harimoto Y, "Expression profiles of genes encoded by the supernumerary chromosome controlling AMtoxin biosynthesis and pathogenicity in the apple pathotype of Alternaria alternata" 20 : 1463-1476, 2007

34 Kunkel BN, "Cross talk between signaling pathways in pathogen defense" 5 : 325-331, 2002

35 Ward ER, "Coordinate gene activity in response to agents that induce systemic acquired resistance" 3 : 1085-1094, 1991

36 Seo PJ, "Cold activation of a plasma membrane-tethered NAC transcription factor induces a pathogen resistance response in Arabidopsis" BLACKWELL PUBLISHING 61 : 661-671, 2010

37 Johnson RD, "Cloning and characterization of cyclic peptide synthetase gene from Alternaria alternata apple pathotype whose product is involved in AM-toxin synthesis and pathogenicity" 13 : 742-753, 2000

38 Matsuoka M, "Classification and structural comparison of full-length cDNAs for pathogenesisrelated proteins" 85 : 942-946, 1987

39 Lagace M, "Characterization of a WRKY transcription factor expressed in late torpedo-stage embryos in Solanum chacoense" 219 : 185-189, 2004

40 Dang ZG, "Changes of several enzymes-activity in apple cultivars inoculated by Alternaria alternata f. sp. mali" 6 : 70-72, 2006

41 Oh SK, "Capsicum annum WRKY protein CaWRKY1 is a negative regulator of pathogen defense" 177 : 977-989, 2008

42 Zheng ZY, "Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens" 48 : 592-605, 2006

43 Linthorst HJ, "Analysis of acidic and basic chitinases from tobacco and petunia and their constitutive expression in transgenic tobacco" 3 : 252-258, 1990

44 Kyte J, "A simple method for displaying the hydropathic character of a protein" 157 : 105-132, 1982

45 van Verk MC, "A novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors" 146 : 1983-1995, 2008