DFO Treatment of HepG2.2.15 Induces Proteomic Changes That May Inhibit Viral Particle Secretion = DFO Treatment of HepG2.2.15 Induces Proteomic Changes That May Inhibit Viral Particle Secretion

다국어 초록 (Multilingual Abstract)

Iron chelators are generally known to have therapeutic value in many diseases. In this study, we investigated the inhibitory effect of drug-induced iron deprivation using DFO (desferrioxamine) on hepatitis B virus (HBV). When HepG 2.2.15 was treated with DFO, cytoplasmic viral DNA concentrations increased without an increase in the secretion of viral particles. Proteomic alternations were observed by two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. 24 proteins showed significant expressional changes. Among those down-regulated, tropomyosin alpha 3 chain may be involved in vesicular transport. Thus, it is believed that its decrease may have affected viral particle secretion. These results indicate that DFO may inhibit the secretion of viral particles. In conclusion, DFO was found to affect a proteomic change in HepG2.2.15 that might inhibit viral secretion.