미토콘드리아 DNA D-loop Region 의 PCR - RFLP 를 이용한 한우 , 제주 재래한우와 타 품종과의 유전적 관계 분석 = Determination of Phylogenetic Relationships of Korean Native and Cheju Native Cattle to Other Breeds Using PCR - RFLP of mtDNA D-loop Region

다국어 초록 (Multilingual Abstract)

This study was conducted to determine molecular genetic differences between Korean native cattle and Cheju native cattle and also investigated phylogenetic relatiqnships to other cattle breeds using PCR-RFLP analysis of mtDNA D-loop region. The cable breeds investigated were Korean native cattle, Cheju native black cattle, Cheju native yellow cattle, Aberdeen Angus, Charolais, Hereford and Holstein. Seventy DNA samples from 7 breeds (10 samples/breed) were examined at 29 sites restricted by 10 enzymes, of which 8 enzymes showed polymorphism when the D-loop region was digested. The 8 enzymes were Msp I, Rsa I, BstX I, Hsp92II, Mbo I, Hxm, Ava II and Hinc II, and on the other hand the two enzymes, Hha I and BamH I, showed no polymorphism. Restriction types and polymorphic sites were used to construct a phylogenetic tree, where Korean native cattle was more closely clustered with Cheju native yellow cattle than the other breeds.