Comparative Ecological Characteristics of Two Potentially Toxic Epiphytic Dinoflagellate Species, Ostreopsis sp. and Coolia canariensis, Native to Jeju Island

다국어 초록 (Multilingual Abstract)

Growth responses along the gradient of water temperature, salinity, and light intensity and cytotoxicity against Artemia nauplii were explored using Ostreopsis sp. and Coolia canariensis strains, representing the two potentially toxic epiphytic dinoflagellate (EPD) species from Jeju coastal waters of Korea.
Variation in maximum growth rate (GRmax) and maximum biomass yield (Ymax) along the environmental gradients was quite contrasting between the two strains, which appears to be reflected in the in situ abundance distribution of the corresponding genera. The more eurythermal characteristics of Ostreopsis sp. strain were in good agreement with the relative distribution of Ostreopsis spp. and Coolia spp. in 520 macroalgal samples collected from 6 stations. The more stenohaline C. canariensis strain was well matched by a markedly narrower range of salinities in the in situ distribution of Coolia spp. than the salinity range for Ostreopsis species. The differences in light adaptation between the high light-preferring Ostreopsis sp. strain and the more euryphotic C. canariensis strain were remarkably consistent with the distinct vertical profiles of Ostreopsis spp. and Coolia spp. abundance in the red alga Amphiroa sp. off Moom-seom. Cytotoxicity against Artemia nauplii in the Ostreopsis sp. preparation with 1000 cells ml-1 was similar to that in C. canariensis preparation with 12000 cells ml-1, which is noteworthy. Thus, the new potential cytotoxicity risks from C. canariensis along with the well-known toxic genus Ostreopsis may be introduced to Jeju coasts, which necessitates further exploration into the contrasting ecological niches occupied by EPD species in relation to their cytotoxicity.

참고문헌 (Reference)

1 백승호, "우리나라 전국연안해역에서 저서 와편모조류의 출현 및 분포현황에 대한 첫 보고" 한국환경생물학회 30 (30): 355-361, 2012

2 백승호, "남해무인도서 백도와 서귀포 인근 섶섬에서 맹독성 저서와편모조류 Gambierdiscus spp.의 출현" 한국해양과학기술원 34 (34): 65-71, 2012

3 Oh MR, "Vertical distribution of epiphytic dinoflagellate in coastal waters of Moon-seom, Jeju Island" Kunsan National University 2015

4 Gémin M, "Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea" 92 : 101727-, 2020

5 Lee KW, "Toxicity of the dinoflagellate Gambierdiscus sp. toward the marine copepod Tigriopus japonicus" 37 : 62-67, 2014

6 Rhodes L, "Toxic marine epiphytic dinoflagellates, Ostreopsis siamensis and Coolia monotis(Dinophyceae), in New Zealand" 34 : 371-383, 2000

7 Park J, "Temporal and spatial distribution of the toxic epiphytic dinoflagellate Ostreopsis cf. ovata in the coastal waters off Jeju Island, Korea" 12 (12): 5864-, 2020

8 Lee SB, "Relative configurational assignment of 4-hydroxyprorocentrolide and prorocentrolide C isolated from a benthic dinoflagellate(Prorocentrum lima)" 82 : 1034-1039, 2019

9 Yih W, "Recent researches on epiphytic dinoflagellates in Korean seas" 2019

10 Md. Mahfuzur Rahman Shah ; 이준백 ; 안소정, "Presence of benthic dinoflagellates around coastal waters of Jeju Island including newly recorded species" 한국생태학회 36 (36): 347-370, 2013

11 Shah, MMR, "Potentiality of benthic dinoflagellate cultures and screening of their bioactivities in Jeju Island, Korea" 13 : 792-805, 2014

12 Yih W, "Population growth and biotoxicity in two newly isolated strains of Korean epiphytic dinoflagellate species" 2011

13 Efimova K, "Phylogenetic characterization of cryptic species of the marine dinoflagellate, Ostreopsis sp. Shmidt, 1902, from Russian coastal waters, the Sea of Japan" 5 (5): 317-332, 2014

14 Hwang BS, "Ostreol A: a new cytotoxic compound isolated from the epiphytic dinoflagellate Ostreopsis cf. ovata from the coastal waters of Jeju Island, Korea" 23 : 3023-3027, 2013

15 이경하 ; 정해진 ; 박기라 ; 강남선 ; 유영두 ; 이무준 ; 이진우 ; 이수진 ; 김태경 ; 김형섭 ; 노재훈, "Morphology and molecular characterization of the epiphytic dinoflagellate Amphidinium massartii, isolated from the temperate waters off Jeju Island, Korea" 한국조류학회I 28 (28): 213-231, 2013

16 Kang, "Morphology and molecular characterization of the epiphytic benthic dinoflagellate Ostreopsis cf. ovata in the temperate waters off Jeju Island, Korea" 27 : 98-112, 2013

17 임안숙 ; 정해진 ; 장태영 ; 강남선 ; 이성연 ; 김형섭 ; 유영두, "Morphology and Molecular Characterization of the Epiphytic Dinoflagellate Prorocentrum cf. rhathymum in Temperate Waters off Jeju Island, Korea" 한국해양과학기술원 48 (48): 1-17, 2013

18 Leung PTY, "Molecular phylogeny and toxicity of harmful benthic dinoflagellates Coolia(Ostreopsidaceae, Dinophyceae)in a sub-tropical marine ecosystem : the first record from Hong Kong" 124 (124): 878-889, 2017

19 Marampouti C, "Mediterranean alien harmful algal blooms : origins and impacts" 28 : 3837-3851, 2021

20 Gladan ŽN, "Massive occurrence of the harmful benthic dinoflagellate Ostreopsis cf. ovata in the Eastern Adriatic Sea" 11 : 300-, 2019

21 Yang AR, "Limaol : a polyketide from the benthic marine dinoflagellate Prorocentrum lima" 80 (80): 1688-1692, 2017

22 Shah MR, "Growth characteristics of three benthic dinoflagellates in mass culture and their antioxidant properties" 11 (11): 268-277, 2016

23 Jang SH, "Gambierdiscus jejuensis sp. nov., an epiphytic dinoflagellate from the waters of Jeju Island, Korea, effect of temperature on the growth, and its global distribution" 80 : 149-157, 2018

24 Jeong HJ, "First report of the epiphytic dinoflagellate Gambierdiscus caribaeus in the temperate waters off Jeju Island, Korea : morphology and molecular characterization" 59 : 637-650, 2012

25 Jeong HJ, "First report of the epiphytic benthic dinoflagellates Coolia canariensis and Coolia malayensis in the waters off Jeju Island, Korea : morphology and rDNA sequences" 59 : 114-133, 2012

26 Selina MS, "First occurrence of the genus Ostreopsis(Dinophyceae)in the Sea of Japan" 53 : 243-249, 2010

27 Chomérat N, "First characterization of Ostreopsis cf. ovata (Dinophyceae) and detection of Ovatoxins during a multispecific and toxic Ostreopsis bloom on French Atlantic coast" 20 (20): 461-, 2022

28 Yoo YD, "Feeding by heterotrophic protists on the toxic dinoflagellate Ostreopsis cf. ovata" 49 : 1-9, 2015

29 Lee B, "Drifting marine plastics as new ecological habitats for harmful eukaryotic microbial communities in Jeju Strait, Korea" 9 : 985756-, 2022

30 Tibiriçá CEJDA, "Diversity and toxicity of the genus Coolia Meunier in Brazil, and detection of 44-methyl gambierone in Coolia tropicalis" 12 (12): 327-, 2020

31 Phua YH, "Diversity and toxicity of Pacific strains of the benthic dinoflagellate Coolia(Dinophyceae), with a look at the Coolia canariensis species complex" 109 : 102120-, 2021

32 Jang B, "Distributional ecology of epiphytic dinoflagellates from the coastal waters off Jeju Island, Korea" Kunsan National University 2013

33 Lee B, "Distribution and genetic diversity of the toxic benthic dinoflagellate genus Ostreopsis in Korea" 96 : 101820-, 2020

34 Hwang BS, "Determination of the absolute configuration of polyhydroxy compound Ostreol B isolated from the dinoflagellate Ostreopsis cf. ovata" 83 : 194-202, 2018

35 Lee SM, "Cytotoxic 4-hydroxyprorocentrolide and prorocentrolide C from cultured dinoflagellate Prorocentrum lima induce human cancer cell death through apoptosis and cell cycle arrest" 12 (12): 304-, 2020

36 Rhodes L, "Coolia monotis(Dinophyceae) : a toxic epiphytic microalgal species found in New Zealand(Note)" 31 : 139-141, 1997

37 An Suk Lim ; 정해진, "Benthic dinoflagellates in Korean waters" 한국조류학회I 36 (36): 91-109, 2021

38 Misurale F, "An integrated in vitro approach for human health and environmental risk assessment of Mediterranean Ostreopsis cf. ovata, Prorocentrum lima, and Coolia monotis strains" 8 : 102-112, 2022

39 김형섭 ; 이원호 ; 오미령 ; 장건강 ; 박종우 ; Yong Deok Ko, "Abundance of Epiphytic Dinoflagellates from Jeju Island during Autumn 2009 Revisited with Special Reference to the Surface-to-Volume Ratio of Substrate Macroalgal Species" 한국해양과학기술원 43 (43): 99-111, 2021

40 김형섭 ; 이원호 ; 김종혁 ; 명금옥 ; 정해진, "Abundance of Epiphytic Dinoflagellates from Coastal Waters off Jeju Island, Korea During Autumn 2009" 한국해양과학기술원 46 (46): 205-209, 2011