발정주기의 소 자궁내막에서 Progesterone이 Prostaglandin 합성효소와 Plasminogen Activator 발현에 미치는 영향

다국어 초록 (Multilingual Abstract)

This study was to investigate effect of progesterone (P4) on prostaglandin (PG) synthases and plasminogen activators (PAs) system in bovine endometrium during estrous cycle. Endometrium tissues were collected from bovine uterus on follicular and luteal phase and were incubated with culture medium containing 0 (Control), 0.2, 2, 20 and 200 ng/ml P4 for 24 h. The PGF2α synthase (PGFS), PGE2 synthase (PGES), cyclooxygenase-2 (COX-2), urokinase PA (uPA), and PA inhibitors 1 (PAI-1) mRNA in bovine endometrium were analyzed using reverse transcription PCR and PA activity was measured using spectrophotometry. In results, COX-2 was higher at 2 ng/ml P4 group than control group in luteal phase (p<0.05), but, it did not change in follicular phase. Contrastively, PGES was significantly increased in 2 ng/ml P4 group compared to control group in follicular phase, but there were no significant differ among the treatments in luteal phase. uPA was no significant difference between P4 treatment groups and control group in both of different phase. PAI-1 was decreased in 20 ng/ml P4 group compared to control group in follicular phase (p<0.05). PA activity was decreased in 2 ng/ml P4 group compared to other groups in follicular and luteal phase (p<0.05). In conclusion, we suggest that P4 may influence to translation and post-translation process of PG production and PA activation in bovine endometrium.

목차 (Table of Contents)

• 서 론
• 재료 및 방법
• 1. 소 자궁내막 조직배양 및 호르몬 처리
• 2. Reverse Transcription PCR
• 3. PA Activity 측정
• 4. 통계 분석
• 결 과
• 1. P4가 PG 합성효소에 미치는 영향
• 2. P4가 PAs 시스템에 미치는 영향
• 고 찰
• 결 론
• REFERENCES