Separation of White Blood Cells in a Wavy Type Microfluidic Device Using Blood Diluted in a Hypertonic Saline Solution

다국어 초록 (Multilingual Abstract)

White blood cells (WBCs) provide crucial information pertaining to human health, and its separation from other blood constituents is imperative for blood-based diagnostics of various pathological conditions. However, efficient WBC separation and enrichment remain to be a challenge. We propose a novel mechanism of WBC separation in a microfluidic device using centrifugal forces, inertial forces, and diluted human blood prepared with hypertonic saline solution. Herein, a simple wavy type microchannel called the separation channel was designed to separate and enrich WBCs. Almost 88% of WBCs were separated with a purity of 83% with diluted blood, i.e., hematocrit (Hct) 2.8% prepared using a 10% hypertonic solution. In addition, the effect of hematocrit and flow rate on WBC separation is also reported in this work. The proposed microdevice can be used for enrichment and separation of WBCs and also develop a better understanding of the interactions amongst blood cells with altered properties.

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