One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher

다국어 초록 (Multilingual Abstract)

One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays.

참고문헌 (Reference)

1 Akiba, H, "Thermodynamics of antibody–antigen interaction revealed by mutation analysis of antibody variable regions" 158 : 1-13, 2015

2 Makabe, K, "Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody" 528 (528): 1156-1166, 2008

3 Bong, J.-H, "Switching- peptides for one-step immunoassay and its application to the diagnosis of human hepatitis B" 178 : 112996-, 2021

4 Maneeprakorn, W, "Surface-enhanced Raman scattering based lateral flow immunochromatographic assay for sensitive influenza detection" 6 : 112079-112085, 2016

5 Swathi, R, "Resonance energy transfer from a dye molecule to graphene" 129 : 054703-, 2008

6 HASANI AMIRHOSSEIN ; Do Ha Huu ; Tekalgne Mahider ; 홍성현 ; 장호원 ; 김수영, "Recent progress of two-dimensional materials and metal–organic framework-based taste sensors" 한국세라믹학회 57 (57): 353-367, 2020

7 Cortijo-Campos, S, "Raman and fluorescence enhancement approaches in graphene-based platforms for optical sensing and imaging" 11 : 644-, 2021

8 Moghaddas S. ; Salehi M. ; Bagheri-Kazemabad S., "Preparation, characterization, and photocatalytic degradation of methylene blue of SnO2/RGO nanocomposite produced by facile hydrothermal process" 한국세라믹학회 59 (59): 698-704, 2022

9 Wiriyachaiporn, N, "Pre-clinically evaluated visual lateral flow platform using influenza A and B nucleoprotein as a model and its potential applications" 11 : 18597-18604, 2021

10 Wu, K, "Portable GMR handheld platform for the detection of influenza a virus" 2 : 1594-1601, 2017

11 봉지홍 ; 김태훈 ; 정재용 ; 이수정 ; 성정수 ; 이창규 ; 강민정 ; 김현옥 ; 변재철, "Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors" 한국바이오칩학회 14 (14): 358-368, 2020

12 Lan, L, "Phase-dependent fluorescence quenching efficiency of MoS2 nanosheets and their applications in multiplex target biosensing" 10 : 42009-42017, 2018

13 Sung, J.S, "One-step immunoassay for food allergens based on screened mimotopes from autodisplayed FVantibody library" 202 : 113976-, 2022

14 Liu, Z, "Near-real-time monitoring of global CO2 emissions reveals the effects of the COVID-19 pandemic" 11 : 1-12, 2020

15 오원춘 ; AREEROB YONRAPACH, "Modeling dye-sensitized solar cells with graphene based on nanocomposites in the Brillouin zone and density functional theory" 한국세라믹학회 58 (58): 50-61, 2021

16 Matson, R.S, "Microarray methods and protocols" CRC Press 2009

17 Wu, K, "Magnetic particle spectroscopy for detection of influenza a virus subtype H1N1" 12 : 13686-13697, 2020

18 Gautier, V, "Lysine conjugation properties in human IgGs studied by integrating highresolution native mass spectrometry and bottom-up proteomics" 15 : 2756-2765, 2015

19 Mayilo, S, "Long-range fluorescence quenching by gold nanoparticles in a sandwich immunoassay for cardiac troponin T" 9 : 4558-4563, 2009

20 Kato, N, "Homogeneous, competitive fluorescence quenching immunoassay based on gold nanoparticle/polyelectrolyte coated latex particles" 109 : 19604-19612, 2005

21 Xie, L, "Graphene as a substrate to suppress fluorescence in resonance Raman spectroscopy" 131 : 9890-9891, 2009

22 Veerapathiran, S, "Fluorescence techniques in developmental biology" 43 : 541-553, 2018

23 Khrenova, M, "Estimating orientation factors in the FRET theory of fluorescent proteins: the TagRFP-KFP pair and beyond" 108 : 126-132, 2015

24 Parker, C.W, "End-to-end protocol for the detection of SARSCoV- 2 from built environments" 5 : e00771-e00820, 2020

25 Zheng, Y, "Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155" 413 : 1605-1614, 2021

26 Li, L.-L, "Direct detection of adenosine in undiluted serum using a luminescent aptamer sensor attached to a terbium complex" 84 : 7852-7856, 2012

27 Goryashchenko, A.S, "Detection of protease activity by fluorescent protein FRET sensors: from computer simulation to live cells" 6 : 022001-, 2018

28 봉지홍 ; 김태훈 ; 정재용 ; 이수정 ; 성정수 ; 이창규 ; 강민정 ; 김현옥 ; 변재철, "Competitive Immunoassay of SARS‑CoV‑2 Using Pig Sera‑Derived Anti‑SARS‑CoV‑2 Antibodies" 한국바이오칩학회 15 (15): 100-108, 2021

29 Toughiri, R, "Comparing domain interactions within antibody Fabs with kappa and lambda light chains." 8 : 1276-1285, 2016

30 Pickering, S, "Comparative performance of SARS-CoV-2 lateral flow antigen tests and association with detection of infectious virus in clinical specimens: a single-centre laboratory evaluation study" 2 : e461-e471, 2021

31 정재용 ; 봉지홍 ; 김홍래 ; 박준희 ; 이창규 ; 강민정 ; 김현옥 ; 변재철, "Anti-SARS-CoV-2 Nucleoprotein Antibodies Derived from Pig Serum with a Controlled Specificity" 한국바이오칩학회 15 (15): 195-203, 2021

32 Vargas-Madrazo, E, "An improved model of association for VH–VL immunoglobulin domains: asymmetries between VH and VL in the packing of some interface residues" 16 : 113-120, 2003

33 Tits, J, "A luminescence line-narrowing spectroscopic study of the uranium (VI) interaction with cementitious materials and titanium dioxide" 44 : 966-976, 2015