항생제 대체 생균제가 자돈의 생산성,영양소 이용률, 유해가스 발생량 및 분내 미생물 수에 미치는 영향 = Effects of Probiotics as an Alternative for Antibiotics on Growth Performance, Nutrient Digestibility, Noxious Gas Emission and Fecal Microbial Population in Growing Piglets

다국어 초록 (Multilingual Abstract)

This study was conducted to determine the effect of probiotics as an alternative for antibiotics on growth performance, nutrient digestibility, noxious gas emission and fecal microbial population in growing piglets. A total of 96 piglets ($22.5{\pm}1.3$kg average body weight) were allotted to 3 different treatment groups and replicated 4 times with 8 piglets per replicate in randomized complete block design. Treatments were T1) (Control, basal diet+0.2% antibiotics), T2) 0.2% probiotics complex and T3) 0.3% Bacillus probiotics. During the whole experiment period, there were no differences (p>0.05) in average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency. However, digestibility of dry matter, crude protein, ether extract, nitrogen free extract and crude ash were showed higher in probiotics groups (T2 and T3) than those of control. In noxious gas emission, ammonia, amine, hydrogen sulfide and mercaptan were significantly (p<0.05) reduced in T2 and T3 treatments compared to those in control. Moisture content of feces was not significantly different among treatments. The colony forming units (CFU) of total bacteria, E. coli and thermoduric bacteria in feces were significantly different among treatments. The CFU of total bacteria, E. coli and thermoduric bacteria in T3 treatment were reduced by feeding probiotics B. From this study, we suggest that probiotics A and B are likely able to improve the growth performance and nutrients digestibility, reduce noxious gas emission and change the fecal microbial composition in growing piglets.

참고문헌 (Reference)

1 조병임, "효모변이균주 유래 β-글루칸과 복합균종 생균제의 혼합급여가 육계의 생산성 및 장내 균총에 미치는 영향" 한국동물자원과학회 50 (50): 89-98, 2008

2 장영달, "항생제 대체제로서 생균제가 이유자돈의 성장능력 및 영양소 이용률, 설사 빈도, 면역 반응에 미치는 영향" 한국동물자원과학회 51 (51): 25-32, 2009

3 배극환, "항생제 대체 기능성 물질의 사용효과에 관한 연구" 41 (41): 23-30, 1999

4 길동용, "지속적인 생균제의 첨가가 돼지의 성장, 영양소 이용율, 혈중 요소태 질소 및 면역능력에 미치는 영향" 한국동물자원과학회 46 (46): 39-48, 2004

5 홍종욱, "자돈 및 비육돈에 있어 생균제의 첨가가 생산성 및 분내 가스 발생에 미치는 영향" 한국동물자원과학회 44 (44): 305-314, 2002

6 한인규, "양돈영양과 사료" 서울대학교 2000

7 석종찬, "생약제제 미라클 ( ？ 첨가가 이유 자돈의 성장률 영양소 이용율 ) , ,분내 미생물균총 및 면역기능에 미치는 영향" 한국동물자원과학회 45 (45): 767-776, 2003

8 김재황, "사료내 발효사료첨가가 비육돈의 생산성 및 분중 암모니아 발생량에 미치는 영향" 43 : 193-202, 2001

9 장해동, "복합 생균제 첨가가 육성돈의 생산성, 면역관련 혈액학적 지표 및 분내 유해가스 발생에 미치는 영향" 한국동물자원과학회 49 (49): 501-508, 2007

10 고영두, "복합 생균제 첨가가 육계 생산성, 유해가스 발생량및 맹장내 균총에 미치는 영향" 한국동물자원과학회 45 (45): 559-568, 2003

11 라정찬, "백년초 혼합 생균제를 이용한 돼지 및 육계에서의 생산성 향상과 환경개선 효과" 28 : 157-167, 2004

12 하종규, "반추동물영양학실험법" 서울대학교출판부 2003

13 Visek, W. J, "The mode of growth promotion by antibiotics" 46 : 1447-1453, 1978

14 Collington, G. K, "The influence of probings or tyrosine on growth of pigs and development the castro-intestial tract" 46 : 521-, 1988

15 Kyriakkis, S. C, "The effect of probiotics LSP 122 on the control post-weaning diarrhoea syndrome of piglets" 67 : 223-228, 1999

16 Callaway, T. R, "The effect of nisin and monensin on ruminal fermentations in vitro" 35 : 90-, 1997

17 Cho, K. H, "The effect of Lactobacillus casei (TSC-66) for growth promotion in piglets (Korean)" 16 (16): 49-53, 1992

18 Han, I. K, "Studies on the growth promoting effects of probiotics. Ⅲ. The effects of Clostridium butyricum ID on the performance and the changes in the microbial flora of the feces of growing pigs" 26 : 166-, 1984

19 SAS Institute,Inc, "SAS user’s guide: Statistics"

20 Newman, C. W, "Replacement of soybean meal in swine diets with L-lysine and Lactobacillus ferments" 37 : 347-, 1988

21 Wenk.C, "Recent Advances in Animal Feed Additives such as metabolic modifiers, antimicrobial agents enzymes and highly available minerals" 13 (13): 86-, 2000

22 Bonomi,A, "Probiotics in pig breeding. Results from the use of Bacillus subtilis and Bacillus licheniformis. Experimental contribution. Rivista-Della-Societa-Italianap-Di-Scienza- Dell" 21 (21): 481-499, 1992

23 HA, CHANG HOON, "Preparation and Analysis of Yeast Cell Wall Mannoproteins, ImmuneEnhancing Materials, from Cell Wall Mutant Saccharomyces cerevisiae" 한국미생물·생명공학회 16 (16): 247-255, 2006

24 AOAC, "Official Method of Analysis (15th ed.)" Association of Official Analytical Chemists 1990

25 Adami, A, "Occurrence of selected bacterial groups in the effects of piglets fed with Bacillus coagulans as probiotic" 39 (39): 3-9, 1999

26 Smith, J. W, "Observation on the alimentary tract and its bacterial flora in healthy and diseased pigs" 86 : 387-, 1963

27 Wenk, C, "Herbs and botanicals as feed additives in monogastric animals" 14-21, 2002

28 Han, I. K, "Growth-stimulating effects of Salinomycin for growing-finishing swine" 24 : 336-, 1982

29 Pollman, D. S, "Effects of microbial feed additives on performance of starter and growing-finishing pigs" 51 : 577-581, 1980

30 Quintero Moreno A, "Effect of probiotics and sex on growth and carcass characteristics of pigs" 6 (6): 5-12, 1996

31 Zani, J. L, "Effect of probiotic CenBiot on the control of diarrhoea and feed efficiency in pigs" 84 (84): 68-71, 1998

32 Noh, S. H, "Effect of dietary growth promoting substances on the growth performance in pig" 37 : 66-, 1995

33 주지환, "Comparison of the Efficacy of Different Organic Acids on Growth Performance and Nutrient Digestibility in Weaned Pigs" 한국동물자원과학회 51 (51): 15-24, 2009

34 Y. T. Ahn, "Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken" 아세아·태평양축산학회 15 (15): 1790-1797, 2002

35 Hosoi, T, "Changes in fecal microflora induced by incubationof mice with Bacillus subtilis(nato) spores are dependent upon dietary components" 45 : 59-, 1999

36 Tagg, G. R, "Bacteriocin of gram-positive bacteria" 40 : 772-, 1976

37 Jack, R. W, "Bacteriocin of gram-positive bacteria" 59 : 171-, 1995

38 Jung K, "Antiviral Effect of Saccharomyces cerevisiae β-glucan to Swine Influenza Virus by Increased Production of Interferon-γ and Nitric Oxide" 51 : 72-76, 2004

39 Otto, E. R, "Ammonia, volatile fatty acids, phenolics and odor offensiveness in manure form growing pigs fed diets reduced in protein concentration" 81 : 1754-1763, 2003

40 Y. Y. Kim, "Acidifier as an Alternative Material to Antibiotics in Animal Feed" 아세아·태평양축산학회 18 (18): 1048-1060, 2005

41 Abuel, S. J, "A study on two types of feed additive as an alternative for antibiotics in swine feeds" 175-, 2006