Acrylamide가 인체상피세포의 발암화에 미치는 영향|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Purpose Acrylamide is present in significant quantities in a wide range of commonly consumed human foods. Carcinogenic risk of acrylamide through the consumption of food is a great public concern and in controversy, but it is not properly addressed due to the lack of evidence in humans. While a plenty of data is available on the carcinogenicity in animal models, the studies in humans are limited. Thus, the present study attempted to examine the carcinogenic potentials of acrylamide on the human epithelial cell, which is the target cell origin of the most cancers. Material and method & Result 1. Acrylamide was not cytotoxic up to $100{\mu}M$ as measured by MTT and LDH assays, indicating a relatively low toxicity of this substance in human epithelial cells. 2. The parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of acrylamide. 3. The neoplastic transformation was further increased with the co-treatment of TPA 4. Antioxidants blocked the generation of Reactive Oxygen Species(ROS) and the GSH depleting agent dramatically increased the ROS production. 5. mRNA levels of fibronectin following acrylamide exposure was increased in a dose-dependent manner, indicating a possible biomarker of acrylamide-induced cellular transformation. Conclusion The present study will provide a valuable basis to compare the interspecies differences in response to carcinogenic potentials of acrylamide. The data on the interspecies differences are essential element in human risk assessment. Thus, our results obtained from the human epithelial cells will contribute to improving the risk assessment of human neoplasm including oral cancer.

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참고문헌 (Reference)

1 Macfarlane GJ, "Trends in survival from cancers of the oral cavity and pharynx in Scotland; a clue as to why the disease is becoming more common?" 73 : 805-808, 1996

2 Farber E, "The multiple nature of cancer" 44 : 4217-4223, 1984

3 Khun K, "Role of nitric oxide, reactive oxygen species, and p38 MAP kinase in the regulation of human chondrocyte apoptosis" 197 : 379-387, 2003

4 Rhim JS, "Neoplastic transformation of human epithelial cells in vitro" 9 : 1345-1366, 1989

5 Rhim JS, "Neoplastic conversion of human epidermal keratinocytes by Ad12-SV40 virus and chemical carcinogens" 232 : 385-388, 1986

6 Marsh GM, "Mortality patterns among workers exposed to acrylamide: 1994 follow up" 56 : 181-190, 1999

7 Hirata Y, "Manganese-induced apoptosis in PC12 cells" 24 (24): 639-653, 2002

8 Lukandu OM, "Khat (Catha edulis) induces reactive oxygen species and apoptosis in normal human oral keratinocytes and fibroblasts" 2008

9 "International Agency for Research on Cancer. Some industrial chemicals" 60 : 389-433, 1994

10 Atsumi T, "Induction of early apoptosis and ROS-generation activity in human gingival fibroblasts (HGF) and human submandibular gland carcinoma (HSG) cells treated with curcumin" 51 (51): 913-921, 2006