Phage 항원성에 미치는 trypsin의 영향 = Effect of trypsin on the phage antigenicity

다국어 초록 (Multilingual Abstract)

In order to investigate the effect of trypsin(TP) on the phage antigenicity, the Klebsiella phage-host cell system and sheep red blood cells were used, and the results were as follows.
The neutralization velocity of Tp-treated phage(Tp phage) by anti-normal phage serum was faster than that of normal phage. And, the neutralization of normal phage by anti-Tp phage serum was remarkably worse than that of Tp phage.
The normal phage - neutralizing activity of the anti-normal phage serum absorbed with Tp phage was very weak. However, the anti-Tp phage serum absorbed with normal phage still considerably neutralized the Tp phage.
The cold phage hemagglutination(CPH) by normal and Tp phage was most favourable when the reactions are carried out with each phage-correspondig antiserum.
The anti-normal phage serum absorbed with Tp phage and the anti-Tp phage serum absorbed with normal phage still formed, with each corresponding phage, the CPH.
The neutralization of Tp phage, by anti-normal phage serum, Passed two times through host bacteria and that of Tp phage, by anti-Tp phage serum, Passed three times through host bacteria were similar to the neutralization of normal phage by each antiserum.
If the Tp phage is passed two times or three times through host bacteria, the forming capability of CPH by each phage bacame similar, under the anti - normal and anti - Tp phage sera, to that of normal phage.
The absorption of Tp phage to host bacteria was a little faster than that of normal phage.
If the Tp tretment of phage is done for 48hours at 37℃, the surviving titer was reduced to around 40%.