Identification of phospholipase C β downstream effect on transient receptor potential canonical 1/4, transient receptor potential canonical 1/5 channels

다국어 초록 (Multilingual Abstract)

Gαq-coupled receptor stimulation was implied in the activation process of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heterotetrameric channels. The inactivation occurs due to phosphatidylinositol 4,5-biphosphate (PI(4,5)P2) depletion. When PI(4,5)P2 depletion was induced by muscarinic stimulation or inositol polyphosphate 5-phosphatase (Inp54p), however, the inactivation by muscarinic stimulation was greater compared to that by Inp54p. The aim of this study was to investigate the complete inactivation mechanism of the heteromeric channels upon Gαq-phospholipase C β (Gαq-PLCβ) activation. We evaluated the activity of heteromeric channels with electrophysiological recording in HEK293 cells expressing TRPC channels. TRPC1/4 and TRPC1/5 heteromers undergo further inhibition in PLCβ activation and calcium/protein kinase C (PKC) signaling. Nevertheless, the key factors differ. For TRPC1/4, the inactivation process was facilitated by Ca2+ release from the endoplasmic reticulum, and for TRPC1/5, activation of PKC was concerned mostly. We conclude that the subsequent increase in cytoplasmic Ca2+ due to Ca2+ release from the endoplasmic reticulum and activation of PKC resulted in a second phase of channel inhibition following PI(4,5)P2 depletion.

참고문헌 (Reference)

1 Storch U, "Transient receptor potential channel 1(TRPC1)reduces calcium permeability in heteromeric channel complexes" 287 : 3530-3540, 2012

2 Myeong J, "The interaction domains of transient receptor potential canonical(TRPC)1/4and TRPC1/5 heteromultimeric channels" 474 : 476-481, 2016

3 Gross SA, "TRPC5 is a Ca2+-activated channel functionally coupled to Ca2+-selective ion channels" 284 : 34423-34432, 2009

4 Tajeddine N, "TRPC1:subcellular localization?" 285 : le1-, 2010

5 Putyrski M, "Switching heterotrimeric G protein subunits with a chemical dimerizer" 18 : 1126-1133, 2011

6 Jeon JP, "Selective Gi subunits as novel direct activators of transient receptor potential canonical(TRPC)4 and TRPC5channels" 287 : 17029-17039, 2012

7 Ong HL, "Role of TRPC channels in store-operated calcium entry" 898 : 87-109, 2016

8 Rohacs T, "Regulation of transient receptor potential channels by the phospholipase C pathway" 53 : 341-355, 2013

9 Mitchell CA, "Regulation of second messengers by the inositol polyphosphate 5-phosphatases" 24 : 994-1000, 1996

10 Venkatachalam K, "Regulation of canonical transient receptor potential(TRPC)channel function by diacylglycerol and protein kinase C" 278 : 29031-29040, 2003

11 Itsuki K, "PLCmediated PI(4, 5)P2 hydrolysis regulates activation and inactivation of TRPC6/7 channels" 143 : 183-201, 2014

12 Nilius B, "Mammalian transient receptor potential (TRP)cation channels" v-vi, 2014

13 Blair NT, "Intracellular calcium strongly potentiates agonist-activated TRPC5 channels" 133 : 525-546, 2009

14 Kinoshita-Kawada M, "Inhibition of TRPC5 channels by Ca2+-binding protein 1 in Xenopus oocytes" 450 : 345-354, 2005

15 Hong C, "Increased TRPC5 glutathionylation contributes to striatal neuron loss in Huntington's disease" 138(Pt 10) : 3030-3047, 2015

16 Shen Y, "G-protein-mediated inhibition of the Trp channel TRPM1 requires the Gβγ dimer" 109 : 8752-8757, 2012

17 Hong C, "Extracellular disulfide bridges stabilize TRPC5 dimerization, trafficking, and activity" 467 : 703-712, 2015

18 Hossain MI, "Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish voltage-sensing phosphatases" 283 : 18248-18259, 2008

19 Storch U, "Dynamic NHERF interaction with TRPC4/5 proteins is required for channel gating by diacylglycerol" 114 : E37-E46, 2017

20 Myeong J, "Dual action of the Gαq-PLCβ-PI(4, 5)P2 pathway on TRPC1/4 and TRPC1/5 heterotetramers" 8 : 12117-, 2018

21 Zhang X, "Direct inhibition of the cold-activated TRPM8 ion channel by Gq" 14 : 851-858, 2012

22 Hofmann T, "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol" 397 : 259-263, 1999

23 Ko J, "Differential PI(4, 5)P2 sensitivities of TRPC4, C5 homomeric and TRPC1/4, C1/5 heteromeric channels" 9 : 1849-, 2019

24 Zhu MH, "Desensitization of canonical transient receptor potential channel 5 by protein kinase C" 289 : C591-, 2005

25 Thakur DP, "Critical roles of Gi/o proteins and phospholipase C-δ1 in the activation of receptor-operated TRPC4 channels" 113 : 1092-1097, 2016

26 Myeong J, "Close spatioassociation of the transient receptor potential canonical 4 (TRPC4)channel with Gαi in TRPC4 activation process" 308 : C879-C889, 2015

27 Dietrich A, "Classical transient receptor potential 1 (TRPC1): channel or channel regulator?" 3 : 939-962, 2014

28 Ordaz B, "Calmodulin and calcium interplay in the modulation of TRPC5channel activity. Identification of a novel C-terminal domain for calcium/calmodulin-mediated facilitation" 280 : 30788-30796, 2005

29 고주연, "Calcium permeability of transient receptor potential canonical (TRPC) 4 channels measured by TRPC4-GCaMP6s" 대한약리학회 21 (21): 133-140, 2017

30 Kim H, "An essential role of PI(4, 5)P₂ for maintaining the activity of the transient receptor potential canonical(TRPC)4β" 465 : 1011-1021, 2013

31 Imai Y, "A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4, 5)P2-diacylglycerol signalling" 590 : 1101-1119, 2012

32 Akbulut Y, "(-)-Englerin A is a potent and selective activator of TRPC4 and TRPC5 calcium channels" 54 : 3787-3791, 2015