LPS로 활성화한 RAW 264.7 세포에서 HK표고버섯균사체의 NF-kB 활성 억제를 통한 항염증 효과 = Anti-inflammatory Efficacy of HK Shiitake Mushroom Mycelium in LPS-treated RAW 264.7 Cells Through Down-regulation of NF-κB Activation

국문 초록 (Abstract)

HK표고버섯균사체(HK shiitake mushroom mycelium, HKSMM)는 간 건강 개별 인정 건강기능식품이다. LPS로 활성화된 RAW 264.7 세포에서 HKSMM50 (HKSMM의 50% ethanol 수용액 추출물)의 항염증효과를 연구하였다. AHCC는 positive control로 사용하였다. LPS로 활성화된 RAW 264.7 세포에 HKSMM50 및 AHCC를 처리(0, 20, 100, 500 μg/ml)하고 24시간 배양하여 배양물의 염증 관련 인자는 ELISA kits로, 세포에 함유된 iNOS와 COX-2 protein 발현은 Western blotting으로 측정하였다. HKSMM50는 LPS 처리에 비해 농도 의존적으로 NF-κB 함량을 낮추었고, iNOS와 COX-2 protein 발현을 억제하여 NO와 PGE2 함량을 낮추었다. 더불어 HKSMM50는 LPS 처리에 비해 IL-1β, TNF-α, IL-4 및 IL-6의 함량을 낮추었으나 SOD와 CAT의 활성은 증가시켰다. AHCC도 HKSSM50 처리와 비슷한 효과를 나타내었다. 이 결과는 HKSMM50이 LPS로 활성화된 RAW 264.7 세포에서 NF-κB 신호전달을 억제하여 항염증효과를 나타내었으며, HKSMM은 면역기능증진에 도움을 줄 수 있는 건강기능식품원료로 사용할 수 있을 것이다.

참고문헌 (Reference)

1 하영래 ; 김영숙 ; 안채린 ; 권정민 ; 박철우 ; 하영권 ; 김정옥, "표고버섯균사체의 사염화탄소 및 알콜로 처리된 흰쥐 간기능 보호 효과" 한국생명과학회 20 (20): 133-141, 2010

2 이준우, "치마버섯 균사체 배양물로부터 분리한 다당류의 항산화 및 면역 활성" 한국식품영양과학회 43 (43): 1334-1341, 2014

3 김다혜 ; 박사라 ; 트리쉬나 ; 하스낫 ; 펄빈 ; 임병우, "노루궁뎅이 버섯 열수추출물의 항산화 활성과 항염증 효능 평가" 한국약용작물학회 21 (21): 112-117, 2013

4 한효상, "꽃송이버섯 추출물이 RAW 264.7 세포에서 TNF-α, iNOS, IL-1β 유전자 발현에 미치는 영향" 대한통합의학회 9 (9): 163-171, 2021

5 Xu, X., "β-Glucan from Saccharomyces cerevisiae reduces lipopolysaccharide-induced inflammatory responses in RAW264.7macrophages" 820 : 1656-1663, 2012

6 Xu, X., "beta-Glucan from Lentinus edodes inhibits nitric oxide and tumor necrosis factor-a production and phosphorylation of mitogen-activated protein kinases in lipopolysaccharide-stimulated murine RAW 264.7 macrophages" 287 : 871-878, 2012

7 Raha, S., "Vitamin D2suppresses amyloid-β 25–35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway" 161 : 37-44, 2016

8 Robaszkiewicz, A., "The role of polyphenols, beta-cartene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms" 2010 : 173274-, 2010

9 Rodrigues, M. L., "The multifunctional fungal ergosterol" 9 : 01755-01718, 2018

10 Taofiq, O., "The contribution of phenolic acids to the anti-inflammatory activity of mushrooms : Screening in phenolic extracts, individual parent molecules and synthesized glucuronated and methylated derivatives" 76 : 821-827, 2015

11 Ministry of Food and Drug Safety, "Standards and specifications of health functional foods. 3-25(237p), 3048(361p) and 3-53(388p)"

12 장욱진 ; 김영숙 ; 하영래 ; 박철우 ; 하영권 ; 김정옥, "Sprague-Dawley 쥐의 사염화탄소 유도 간 손상에 대한 표고버섯균사체의 효과" 한국생명과학회 20 (20): 782-788, 2010

13 Guzik, T. J., "Nitric oxide and superoxide in inflammation and immune regulation" 54 : 469-487, 2003

14 Trianthi, P., "NF-kB transcription.. factor: a key player in the generation of immune response" 90 : 519-531, 2006

15 Liu, T., "NF-kB signaling in inflammation" 2 : e17023-, 2017

16 Garcia-Lafuente, A., "Mushrooms as a source of anti-inflammatory agents & anti-allergy agents" 9 : 125-141, 2010

17 Sajon, S. R., "Mushrooms : Natural factory of anti-oxidant, anti-inflammatory, analgesic and nutrition" 7 : 464-475, 2018

18 Du, C., "Mediators of inflammation : Inflammation in cancer, chronic diseases, and wound healing" 2015 : 570653-, 2015

19 Mann, J. R., "Mechanisms of disease : Inflammatory mediators and cancer prevention" 2 : 202-210, 2005

20 Liu, Y. C., "Macrophage polarization in inflammatory diseases" 10 : 520-529, 2014

21 Martinez, F. O., "Macrophage activation and polarization" 13 : 453-461, 2008

22 Carbonero, E. R., "Lentinus edodes heterogalactan : Antinociceptive and anti-inflammatory effects" 111 : 531-537, 2008

23 Bisen, P. S., "Lentinus edodes : A macrofungus with pharmacological activities" 17 : 2419-2430, 2010

24 Crespo, H., "Lentinula edodes β-glucan enriched diet induces pro-and anti-inflammatory macrophages in rabbit" 61 : 141279-, 2017

25 Parola, S., "Lentinula edodes and Pleurotus ostreatus : functional food with antioxidant-antimicrobial activity and an important source of Vitamin D and medicinal compounds" 7 : 773-794, 2017

26 Fiorucci, S., "Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa" 100 : 10937-10941, 2003

27 Libby, P., "Inflammation and atherosclerosis" 105 : 1135-1143, 2002

28 Nagappan, A., "Indomethacin inhibits activation of endothelial nitric oxide synthase in the rat kidney : possible role of this effect in the pathogenesis of indomethacininduced renal damage" 25 : 77-87, 2014

29 Park, Y. M., "In vivo and in vitro anti-inflammatory and anti-nociceptive effects of the methanol extract of Inonotus obliquus" 101 : 120-128, 2005

30 Ministry of Food and Drug Safety, "HK shiitake mushroom mycelium. Certificate of health functional food ingredient for liver health. 2010-35"

31 Kuo, M. C., "Ganoderma lucidum mycelia enhance innate immunity by activating NF-κB" 103 : 217-222, 2006

32 Yoon, H. M., "Ganoderma lucidum ethanol extract inhibits the inflammatory response by suppressing the NF-κB and toll-like receptor pathways in lipopolysaccharide-stimulated BV2 microglial cells" 5 : 957-963, 2013

33 Park, S. O., "Functional food for immune regulation-beta-glucan" 45 : 39-47, 2012

34 Kim, J. I., "Efficacy of the blood lipid improvement, antioxidant, and liver function improvement of shiitake mushroom mycelium powder in the high alcohol intake group" Inje University 2009

35 Takanari, J., "Effects of active hexose correlated compound on the seasonal variations of immune competence in healthy subjects" 20 : 28-34, 2015

36 Lin, J. T., "Chemical composition, antioxidant and anti-inflammatory properties for ethanolic extracts from Pleurotus eryngii fruiting bodies harvested at different time" 55 : 374-382, 2014

37 Gunawardena, D., "Anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages" 148 : 92-96, 2014

38 Chien, R. C., "Anti-inflammatory effects of extracts from the medicinal mushrooms Hypsizygus marmoreus and Pleurotus eryngii(Agaricomycetes)" 18 : 477-487, 2016

39 Van, Q., "Anti-inflammatory effect of Inonotus obliquus, Polygala senega L., and Viburnum trilobum in a cell screening assay" 125 : 487-493, 2009

40 Ma, L., "Anti-inflammatory and anticancer activities of extracts and compounds from the mushroom Inonotus obliquus" 139 : 503-508, 2013

41 Jedinak, A., "Anti-inflammatory activity of edible oyster mushrooms is mediated through the inhibition of NF-kB and AP-1 signaling" 10 : 52-, 2011

42 서경혜 ; 박정용 ; 노형준 ; 이지연 ; 이은영 ; 한재구 ; 김진효 ; 정미선, "Anti-inflammatory Effects of Various Mushrooms in LPS-stimulated RAW264.7 Cells" 한국자원식물학회 31 (31): 478-488, 2018

43 Du, B., "An insight into anti-inflammatory effects of fungal beta-glucan" 41 : 49-59, 2015

44 Finimundy, T. C., "A review on general nutritional compounds and pharmacological properties of the Lentinula edodes mushroom" 5 : 1095-1105, 2014