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      Human hnRNP L shuttles between Nucleus and cytoplasm

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      https://www.riss.kr/link?id=E805452

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      The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domains. Recently published data suggest that hnRNP L plays roles on cytoplasmic functions, such as mRNA turnover and regulation of translation. In this study, we investigated the nucleo-cytoplasmic shuttling activity of hnRNP L using transient transfection in conjunction with interspecies heterokaryon assays. Here, we report that hnRNP L, although it is localized primarily in the nucleoplasm, shuttles between nucleus and cytoplasm. The hnRNP L protein is detected mainly in the nucleoplasm by immunofluorescence microscopy, presumably because the return of hnRNP L to the nucleus is very rapid.
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      The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domai...

      The heterogeneous nuclear ribonucleoprotein L (hnRNP L) is an abundant nuclear protein and one of the major pre-mRNA binding proteins. It is a 68kD protein and contains 558 amino acid residues and four loosely conserved RNP-concensus RNA-binding domains. Recently published data suggest that hnRNP L plays roles on cytoplasmic functions, such as mRNA turnover and regulation of translation. In this study, we investigated the nucleo-cytoplasmic shuttling activity of hnRNP L using transient transfection in conjunction with interspecies heterokaryon assays. Here, we report that hnRNP L, although it is localized primarily in the nucleoplasm, shuttles between nucleus and cytoplasm. The hnRNP L protein is detected mainly in the nucleoplasm by immunofluorescence microscopy, presumably because the return of hnRNP L to the nucleus is very rapid.

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      목차 (Table of Contents)

      • INTRODUCTION
      • MATERIALS AND METHODS
      • Plasmid constructions and preparation
      • Cell culture and transfection
      • Heterokaryon formation
      • INTRODUCTION
      • MATERIALS AND METHODS
      • Plasmid constructions and preparation
      • Cell culture and transfection
      • Heterokaryon formation
      • Immunofluorescence microscopy
      • RESULTS AND DISCUSSION
      • REFERENCES
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