A Histone Deacetylase, MoHDA1 Regulates Asexual Development and Virulence in the Rice Blast Fungus|RISS 상세보기

다국어 초록 (Multilingual Abstract)

Interplay between histone acetylation and deacetylation is one of the key components in epigenetic regulation of transcription. Here we report the requirement of Mo- HDA1-mediated histone deacetylation during asexual development and pathogenesis for the rice blast fungus, Magnaporthe oryzae. Structural similarity and phylogenetic analysis suggested that MoHDA1 is an ortholog of Saccharomyces cerevisiae Hda1, which is a representative member of class II histone deacetylases. Targeted deletion of MoHDA1 caused a little decrease in radial growth and large reduction in asexual sporulation. Comparison of acetylation levels for H3K9 and H3K14 showed that lack of MoHDA1 gene led to significant in- crease in H3K9 and H3K14 acetylation level, compared to the wild-type and complementation strain, confirm- ing that it is a bona fide histone deacetylase. Expression analysis on some of the key genes involved in asexual reproduction under sporulation-promoting condition showed almost no differences among strains, except for MoCON6 gene, which was up-regulated more than 6-fold in the mutant than wild-type. Although the deletion mutant displayed little defects in germination and subsequent appressorium formation, the mutant was compromised in its ability to cause disease. Wound- inoculation showed that the mutant is impaired in invasive growth as well. We found that the mutant was defective in appressorium-mediated penetration of host, but did not lose the ability to grow on the media containing H2O2. Taken together, our data suggest that MoHDA1-dependent histone deacetylation is important for efficient asexual development and infection of host plants in M. oryzae.

번역하기

참고문헌 (Reference)

1 Choi, J., "dbHiMo: a web-based epigenomics platform for histone-modifying enzymes" 2015 : bav052-, 2015

2 Wilson, R. A, "Under pressure: investigating the biology of plant infection by Magnaporthe oryzae" 7 : 185-195, 2009

3 Ding, S. -L., "The tig1 histone deacetylase complex regulates infectious growth in the rice blast fungus Magnaporthe oryzae" 22 : 2495-2508, 2010

4 Srikantha, T., "The histone deacetylase genes HDA1 and RPD3 play distinct roles in regulation of high-frequency phenotypic switching in Candida albicans" 183 : 4614-4625, 2001

5 Dean, R., "The Top 10fungal pathogens in molecular plant pathology" 13 : 414-430, 2012

6 Yang, X.-J, "The Rpd3/Hda1 family of lysine deacetylases: from bacteria and yeast to mice and men" 9 : 206-218, 2008

7 Mulder, N. J, "The InterPro database and tools for protein domain analysis" 2008

8 Elias-Villalobos, A., "The Hos2 histone deacetylase controls Ustilago maydis virulence through direct regulation of mating-type genes" 11 : e1005134-, 2015

9 Li, Y., "The HDF1 histone deacetylase gene is important for conidiation, sexual reproduction, and pathogenesis in Fusarium graminearum" 24 : 487-496, 2011

10 Wu, J., "TUP1 utilizes histone H3/H2B-specific HDA1 deacetylase to repress gene activity in yeast" 7 : 117-126, 2001