백서두개골 결손부에서 BMP전달체의 골재생효과분석 = The Analysis of Bone regenerative effect with carriers of bone morphogenetic protein in rat calvarial defects

다국어 초록 (Multilingual Abstract)

Bone morphogenetic proteins have been shown to possess significant osteoinSductive potential, but in order to take advantage of this effect for tissue engineering, carrier systems are essential. Successful carrier systems must enable vascular and cellular invasion, allowing BMP to act as a differentiation factor. The carrier should be reproducible, non-immunogenic, moldable, and space-providing, to define the contours of the resulting bone. The purpose of this study was to review available literature, in comparing various carriers of BMP on rat calvarial defect model. The following conclusions were deduced. 1. Bone regeneration of ACS/BMP, ${\beta}-TCP/BMP$, FFSS/BMP, $FFSS/{\beta}-TCP/BMP$, MBCP/BMP group were significantly greater than the control groups. 2. Bone density in the ACS/BMP group was greater than that in ${\beta}-TCP$, FFSS, $FFSS/{\beta}-TCP$ carrier group. 3. Bone regeneration in FFSS/BMP group was less than in ACS/BMP, ${\beta}-TCP/BMP$, MBCP/BMP group. However, New bone area of $FFSS/{\beta}-TCP/BMP$ carrier group were more greater than that of FFSS/BMP group. ACS, ${\beta}-TCP$, FFSS, $FFSS/{\beta}-TCP$, MBCP were used for carrier of BMP. However, an ideal carrier which was reproducible, non-immunogenic, moldable, and space-providing did not exist. Therefore, further investigation are required in developing a new carrier system.

참고문헌 (Reference)

1 "Urist MR. Human fibrin is a physiologic delivery system for bone morphogenetic protein. Clin Orthop Relat Res 1988" 302-310,

2 "Two distinctive BMP-carriers induce zonal chondrogenesis and membranous ossification, respectively; geometrical factors of matrices for cell-differentiation" 32 (32): 219-226, 1995

3 "Turek T et al. Osseous regeneration in the rat calvarium using novel delivery systems for recombinant human bone morphogenetic protein-2" 1139-2 1147,

4 "Thies RS. The BMP proteins in bone formation and repair. Trends Genet 1992" 97-102,

5 "The effect of varying the particle size of beta tricalcium phosphate carrier of recombinant human bone morphogenetic protein-4 on bone formation in rat calvarial defects" 77 (77): 765-772, 2006

6 "The effect of recombinant human bone morphogenetic protein-4 on the osteoblastic differentiation of mouse calvarial cells affected by Porphyromonas gingivalis" 73 (73): 1126-1132, 2002

7 "The effect of recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system on bone formation in rat calvarial defects" 37 : 397-408, 2007

8 "The effect of a fibrin-fibronectin/beta-tricalcium phosphate/recombinant human bone morphogenetic protein-2 system on bone formation in rat calvarial defects" 27 (27): 3810-3816, 2006

9 "Reddi AH. Isolation of osteogenin" 7109-7113,

10 "Reddi AH. Importance of geometry of the extracellular matrix in endochondral bone differentiation. J Cell Biol 1984" 2192-2197,

11 "Periodontal repair in dogs: evaluation of rhBMP-2 carriers" 16 (16): 524-537, 1996

12 "Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment" 26 (26): 392-400, 1999

13 "Ozawa H. Enhanced osteoinduction by intramuscular grafting of BMP-beta-TCP compound pellets into murine models. Arch Histol Cytol 1992" 97-112,

14 "Ono K. Bone induction in monkeys by bone morphogenetic protein. A trans-filter technique. J Bone Joint Surg Br 1993" 107-110,

15 "Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res 1986" 299-308,

16 "Hightower JA. The matrix of endochondral bone differs from the matrix of intramembranous bone. Calcif Tissue Int 1991" 349-354,

17 "Factors that modulate the effects of bone morphogenetic protein-induced periodontal regeneration: a critical review" 73 (73): 925-936, 2002

18 "Evaluation of human recombinant bone morphogenetic protein-2-loaded tricalcium phosphate implants in rabbits' bone defects" 25 : 55S-58S, 1999

19 "Enhanced healing of segmental tibial defects in sheep by a composite bone substitute composed of tricalcium phosphate cylinder, bone morphogenetic protein, and type IV collagen" 32 (32): 505-512, 1996

20 "Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects" 74 (74): 787-797, 2003

21 "Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in a rat calvarial defect model" 75 (75): 1364-1370, 2004

22 "Effect of recombinant human bone morphogenetic protein-2/absorbable collagen sponge (rhBMP -2/ACS) on healing in 3-wall intrabony defects in dogs" 73 (73): 63-72, 2002

23 "Effect of recombinant human bone morphogenetic protein-2, -4, and -7 on bone formation in rat calvarial defects" 76 (76): 1667-1674, 2005

24 "Effect of a fibrin-fibronectin sealing system as a carrier for recombinant human bone morphog enetic protein-4 on bone formation in rat calvarial defects" 76 (76): 2216-2222, 2005

25 "Ectopic bone formation associated with recombinant human bone morphogenetic proteins-2 using absorbable collagen sponge and beta tricalcium phosphate as carriers" 26 (26): 2501-2507, 2005

26 "Cunningham NS. Bone induction by osteogenin and bone morphogenetic proteins. Biomaterials 1990" 33-34,

27 "Clauser C. Fibrin and fibronectin sealing system in a guided tissue regeneration procedure. A case report. J Periodontol 1988" 679-683,

28 "Bone morphogenetic proteins in human bone regeneration" 142 (142): 9-21, 2000

29 "Bone morphogenetic proteins" 22 (22): 233-241, 2004

30 "Amitani K et al. Solubilization and concentration of a bone-inducing substance from a murine osteosarcoma. Clin Orthop Relat Res 1980" 274-280,

31 "-induced repair of skull trephine defects in pigs. Clin Orthop Relat Res 1994" 263-270,