LC-MS/MS를 이용한 진피 지표물질의 함량분석 = Quantitative Content Analysis of Analytical Marker in Citrus unshiu peel by LC-MS/MS

다국어 초록 (Multilingual Abstract)

Getting the spotlight to traditional medicine recently, the number of adverse effect and drug-drug interaction of traditional medicine is increased. For this reason, pharmacokinetics study of traditional medicine need to scientific prove of safety and effectiveness. The method of pharmacokinetics study of traditional medicine, Botanical drug guideline of U.S. FDA recommend to measure analytical marker concentration in blood. The aim of this study is quantitative content analysis of analytical marker in citrus unshiu peel for help pharmacokinetics study of traditional medicine. We seleceted naringin, naringenin, hesperidin and hesperetin as analytical marker and used HPLC-MS/MS as analysis equipment. Internal standard (IS) is domperidone. The mass transition of hesperidin, hesperetin, naringin and naringenin were 609.4 → 300.9, 301.0 → 285.8, 579.23 → 270.9 and 271.0 → 150.8 respectively. The result of this study is that 1g citrus unshiu peel has 12.24 mg (1.224%) hesperidin, 0.66 mg (0.066%) hesperetin, 15.17 mg (1.517%) naringin, and naringenin was not detected. citrus unshiu peel analytical marker that we can detect is naringin, naringenin, hesperidin. We could confirmed amount of citrus unshiu peel analytical marker through this study and our data further support the pharmacokinetics study of traditional medicine.