Mixed starter of Lactococcus lactis and Leuconostoc citreum for extending kimchi shelf-life

다국어 초록 (Multilingual Abstract)

To develop a starter culture system for improving the shelflife and quality of kimchi, we prepared a mixed starter composed of Lactococcus lactis and Leuconostoc citreum. Two strains, L. lactis WiKim0098 and Leu. citreum WiKim0096, showed high antimicrobial activity and mannitol productivity, respectively. These lactic acid bacteria (LAB) were introduced as a starter into kimchi following cultivation in foodgrade liquid medium. Two kimchi samples, with and without starter, were fermented for 12 days at 10°C. Compared to the control kimchi without starter, a lower initial pH and higher number of LAB were observed in kimchi with starter at 0 day. However, the starter in kimchi prolonged the period taken by kimchi to reach to pH 4.2 by approximately 1.5-fold compared to that in the control kimchi. To estimate the effect of the starter on the flavor of kimchi, metabolite changes were evaluated by gas chromatography/mass spectrometry. In starter fermented kimchi, the levels of mannitol and amino acid, which are associated with the flavor of kimchi, were increased following fermentation. The amount of mannitol was confirmed by high-performance liquid chromatography analysis, showing concentrations of 3.4 and 5.1 mg/ml for the control and starter fermented kimchi, respectively. Thus, mixed starter inoculated with L. lactis WiKim0098 and Leu. citreum WiKim0096 may extend the shelf-life of kimchi and improve its sensory characteristics.

참고문헌 (Reference)

1 김효주, "혼합 스타터를 이용한 묵은지의 제조 및 발효 특성" 한국식품영양과학회 42 (42): 1467-1474, 2013

2 최학종, "김치의 저장성 향상을 위한 항균활성 우수 유산균 선발" 한국식품영양과학회 43 (43): 328-332, 2014

3 Kang, B. K., "The influence of red pepper powder on the density of Weissilla koreensis during kimchi fermentation" 5 : 15445-, 2015

4 이모은, "Starter Cultures for Kimchi Fermentation" 한국미생물·생명공학회 25 (25): 559-568, 2015

5 Jung, M. Y., "Role of jeotgal, a Korean traditional fermented fish sauce, in microbial dynamics and metabolite profiles during kimchi fermentation" 265 : 135-143, 2018

6 Sadiq, S., "Potential of bacteriocinogenic Lactococcus lactis subsp. lactis inhabiting low pH vegetables to produce nisin variants" 59 : 204-210, 2014

7 문송희, "Pichia kudriavzevii is the Major Yeast Involved in Film-formation, Off-odor Production, and Texture-softening in Over-ripened Kimchi" 한국식품과학회 23 (23): 489-497, 2014

8 Lane, D. J., "Nucleic acid techniques in bacterial systematics" Wiley 115-175, 1991

9 Lee, K., "Mixed cultures of kimchi lactic acid bacteria show increased cell density and lactate productivity" 12 : 4000-4005, 2013

10 Jeong, S. H., "Microbial succession and metabolite changes during long-term storage of kimchi" 78 : 763-769, 2013

11 Chong, J., "MetaboAnalyst 4. 0 : towards more transparent and integrative metabolomics analysis" 46 : W486-W494, 2018

12 Otgonbayar, Gan-Erdene, "Mannitol Production by Leuconostoc citreum KACC 91348P Isolated from Kimchi" 한국미생물·생명공학회 21 (21): 968-971, 2011

13 Jung, J. Y., "Kimchi microflora : history, current status, and perspectives for industrial kimchi production" 98 : 2385-2393, 2014

14 Chang, J. Y., "Improvements in the quality and shelf life of kimchi by fermentation with the induced bacteriocin-producing strain, Leuconostoc citreum GJ7 as a starter" 75 : 103-110, 2010

15 장자영, "Extending the Shelf life of Kimchi with Lactococcus lactis Strain as a Starter Culture" 한국식품과학회 24 (24): 1049-1053, 2015

16 Kim, D. W., "Effects of different salt treatments on the fermentation metabolites and bacterial profiles of kimchi" 82 : 1124-1131, 2017

17 Jung, J. Y., "Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during kimchi fermentation" 153 : 378-387, 2012

18 Jo, S. Y., "Characterization of starter kimchi fermented with Leuconostoc kimchii GJ2 and its cholesterol-lowering effects in rats fed a high-fat and highcholesterol diet" 95 : 2750-2756, 2015

19 Park, S. E., "Changes of microbial community and metabolite in kimchi inoculated with different microbial community starters" 274 : 558-565, 2019