임상검체에서 분리한 황색포도상 구균의 항생제 Tolerance에 관한 연구 = Antibiotic Tolerance of Staphylococcus aureus Isolated form Various Clinical Speciemens

다국어 초록 (Multilingual Abstract)

Cell-wall inhibitor can rapidly kill and many case lyse susceptible bacteria. But in tolerant strain, bactericidal antibiotics act primarily as bacteristatic agents. Determination of minimal bactericidal concentration(MBC) of antibiotics is important because infection in immunocompromised host may require bactercidal rather than bacteriostatic antibiotic level.
Thirty-five Staphhlococcus aureus were isolated from various clinical specimens and MBC of cephalothin, gentamicin, methicillin, vancomycin was determined by macobroth dilution method. Time-kill curve was made by counting survior at 0, 4, 8, 24h of incubaton in minimal inhibitory corcentration(MIC) of antibiotics.
By NCCLS criteria, 22, 13, 24, 32 stains of 35 S. aureus were susceptible to cephalothin, gentamicin, methicillin and vancomycin, respectively. Among the suscoptible strains, tolorance rate of each antibiotics were 10/22, 0/13, 14/24, 13/32, respectivly when S. aureus was defined as being tolerant if the ratio of MBC/MIC is greater than or equal to 32. By time-kill curve, 15/22, 1/13, 12/24, 12/32, were tolerant to cephalothin, gentamicin, methicillin and vancomycin, respectively. By MBC/MIC and time-kill curve method, 17/22, 12/13, 20/24, 23/32 showed the same tolerance to cephalothin, gentamicin, methicillin and vancomycin, respectively. The climical significance of these tolerant strains of S. aureus couldn't be evaluated in this study.