배추와 양배추 추출물의 생리활성 물질 분석 및 암세포 증식 억제효과 = Determination of Bioactive Compounds and Anti-cancer Effect from Extracts of Korean Cabbage and Cabbage

다국어 초록 (Multilingual Abstract)

In this study, we determined total polyphenol content(TPC) and total flavonoid content(TFC) of extracts from Korean cabbage and cabbage using a spectrophotometric method as well as glucosinolates concentration by HPLC. TPCs of Korean cabbage and cabbage extracts were 308.48 ㎍ GAE/g dry weight and 344.75 ㎍ GAE/g dry weight, respectively. TFCs of Korean cabbage and cabbage extracts were 5.33 ㎍ QE/g dry weight and 5.95 ㎍ QE/g dry weight, respectively. We found six different glucosinolates, namely progoitrin, glucoalyssin, gluconapin, glucobrassicanapin, glucobrassicin and 4-methoxyglucobrassicin in the Korean cabbage extract. In the cabbage extract, there was four glucosinolates, namely glucoraphanin, sinigrin, glucobrassicin and 4-methoxyglucobrassicin. We determined the cytotoxic effect of Korean cabbage and cabbage extracts in AGS human stomach cancer cells, HepG2 human hepatic cancer cells and LNCaP human prostate cancer cells by MTT assay. Dose-dependent relationships were found between the extract concentrations and cancer cell growth inhibition. The overall results support that both Korean cabbage and cabbage, the major vegetables in Korea, contain bioactive compounds such as polypheol, flavonoids as well as glucosinolates and they may play a positive role in cancer prevention.

국문 초록 (Abstract)

본 연구에서는 배추와 양배추 에탄올 추출물의 총 폴리페놀 함량 및 총 플라보노이드 함량을 측정하고, glucosinolates 함량을 HPLC로 분석하였다. 또한, MTT assay를 통한 암세포증식 억제 활성을 측정하였다. 배추와 양배추 에탄올 추출물의 항산화물질로 알려진 총 폴리페놀 함량은 각각 308.48 및344.75 ㎍ GAE/g dry weight으로 나타났으며, 총 플라보노이드 함량은 각각 5.33 및 5.95 ㎍ QE/g dry weight으로 나타났다.
배추 추출물에서는 progoitrin, glucoalyssin, gluconapin, glucobrassicanapin,glucobrassicin, 4-methoxyglucobrassicin의 총 6개glucosinolates를 확인하였다. 양배추 추출물에서는 glucoraphanin,sinigrin, glucobrassicin 및 4-methoxyglucobrassicin의 총 4개glucosinolates를 확인하였다. 배추와 양배추 에탄올 추출물이AGS 인체 위암세포주, HepG2 인체 간암세포주, LNCaP 인체전립선암 세포주 증식에 미치는 영향을 MTT assay를 통해 알아보았다. 배추와 양배추 추출물의 농도가 증가함에 따라 농도 의존적으로 암세포 증식 억제 효능이 증가하였다. 또한,배추와 양배추 추출물을 암세포에 처리하고 배양하는 시간이 24시간에서 48시간으로 길어질수록 암세포 성장 억제 효능도 증가하였다. 배추와 양배추의 추출과정 중에 생성된glucosinolate 가수분해 산물과 폴리페놀, 플라보노이드 등의 생리활성 물질들이 암세포 성장 억제에 직접적인 영향을주었을 것으로 사료된다. 한국인들이 자주 섭취하고 있는배추와 양배추에는 주요 생리활성 물질인 글루코시놀레이트뿐 아니라 폴리페놀, 플라보노이드가 함유되어 있으며,이들 추출물들은 암세포 증식 억제 효능이 있음을 보여주었다.

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