N-Isopropylacrylamide-기반 나노구조체를 활용한 온도감응성 항생제 용출 콘택트렌즈 개발 = Development of Thermo-Responsive Antibiotic-Eluting Contact Lenses Using N-Isopropylacrylamide-Based Nanostructures

다국어 초록 (Multilingual Abstract)

Purpose : We prepared antibiotic-eluting thermo-sensitive nanostructures in order to manufacture smart contact lenses in which the antibiotic is not eluted during storage and the antibiotic is eluted only when worn on the eye.
Methods : Emulsion polymerization was performed to synthesize p(NIPAAm)-based nanostructures, which were introduced into contact lenses through a sandwich method. The antibiotic levofloxacin (LVF) was loaded on the contact lens through the soaking method, and the antibiotic elution was analyzed according to the temperature.
Results : Temperature-sensitive p(NIPAAm)-based nanostructures with a size of 20-40 nm were synthesized through emulsion polymerization using sodium n-dodecyl sulfate (SDS) micelle templates, which were confirmed by TEM and particle size analysis. The nanostructures were introduced into the contact lens through the sandwich method, and the antibiotic was loaded into the nanostructures inside the lens through the soaking method. The release profiles of antibiotics were analyzed at 25 oC and 35 oC, respectively. At room temperature, the antibiotic was released within 3 ug, but at 35 oC most of the antibiotic was released within 2 hours and up to 10 ug was released.
Conclusion : In this study, thermo-sensitive smart antibiotic-eluting contact lenses were manufactured by synthesizing thermo-sensitive nanostructures, applying it to a contact lens, and loading an antibiotic. The thermo-sensitive nanostructures were able to successfully load antibiotics in the contact lens, stored a considerable amount of antibiotics at room temperature, and released a large amount of antibiotics up to 10 μg when the temperature increased. The results of this study are expected to play a significant role in the development and commercialization of smart ophthalmic medical devices and contact lenses for drug delivery.

국문 초록 (Abstract)

목적 : 온도감응성 나노구조체를 제조하고 이를 적용하여, 보관시에는 항생제가 용출되지 않고, 안구착용시에만 온도감응성으로 항생제를 용출하는 스마트 콘택트렌즈를 제조하고자 한다.
방법 : 에멀젼중합하여 p(NIPAAm)-기반의 나노구조체를 합성하였고, 이를 샌드위치 공법을 통해 콘택트렌즈에 도입하였다. Soaking 방법을 통해 항생제인 levofloxacin(LVF)를 콘택트렌즈에 탑재하여 온도에 따라 항생제용출 특성을 분석하였다.
결과 : sodium n-dodecyl sulfate (SDS) 마이셀 템플레이트를 활용한 에멀젼중합을 통해 20-40 nm 크기의온도감응형 p(NIPAAm)-기반의 나노구조체를 합성하였고 이는 TEM과 입도분석기를 통해 확인하였다. 샌드위치공법을 통해 콘택트렌즈에 나노구조체를 도입하였고, soaking 방법을 통해 항생제를 렌즈안의 나노구조체에 탑재하였다. 25 oC와 35 oC에서 각각 항생제의 방출 특성을 분석하였다. 상온에서는 항생제를 3 ug 이내로 방출하였지만, 35 oC에서는 2시간이내에 대부분의 항생제를 방출하였고 10 ug까지 방출하였다.
결론 : 본 연구에서는 온도감응형 나노구조체를 합성하고, 이를 콘택트렌즈에 적용 및 항생제를 탑재하여, 온도감응형 스마트 항생제용출 콘택트렌즈를 제조하였다. 온도감응형 나노구조체는 콘택트렌즈안에서 항생제를 성공적으로 탑재할 수 있었고, 상온에서 상당량의 항생체를 보관하고, 온도증가시 10 ug까지의 많은 양의 항생제를방출하였다. 본 연구결과는 약물전달용 스마트 안과의료기기 및 콘택트렌즈의 개발 및 상용화에 큰 역할을 할 것으로 기대된다.

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