한국인에서 과립구항원 HNA-3 유전자 빈도 = Gene Frequency of Human Neutrophil Alloantigen-3 in the Korean Population

국문 초록 (Abstract)

배경: 한국인의 Human Neutrophil Alloantigen-1 (HNA-1), HNA-2, HNA-4, HNA-5 유전자 빈도에대한 연구는 혈청학적 혹은 분자유전적 방법을이용하여 이미 이루어졌으나 HNA-3의 유전자 빈도는 아직 연구되지 않았다. 저자들은 이번 연구에서 한국인의 HNA-3a의 유전자빈도를 확인하여 HNA-3a (HNA-3b) 동종면역위험률과 HNA-3 부적합률을 계산하고 HNA-3항체검사에 사용할과립구를 제공할 공혈자를 확보하고자 하였다.
방법: 저자들은 110명의 건강한 비혈연 한국인공혈자의 HNA-3유전자를 염기서열특이-중합효소연쇄반응 이용하여 검사하였다.
결과: 한국인의 HNA-3a, HNA-3b 유전자 빈도는 각각 0.695와 0.305였다. 이 중 HNA-3a유전자빈도가 미국 백인, 독일인보다 통계적으로 유의하게 낮았다(P＜0.05). 임신(수혈)에 의한 HNA-3a 동종면역위험률과 HNA-3b 동종면역위험률은 각각0.065 (0.084), 0.147 (0.250)였고 한국인의 HNA-3a 동종면역위험률은 미국 백인, 독일인에 비해 유의하게 높았다(P＜0.05).
결론: 저자들은 한국인에서 HNA-3 유전자 빈도, 임신 혹은 수혈에 의한 HNA-3a 동종면역위험률과 HNA-3b동종면역위험률을 확인하였고HNA-3 항체검사에 사용할 과립구 패널에 HNA-3a/3a, HNA-3b/3b 과립구를 제공할 공혈자를 확인하였다.

다국어 초록 (Multilingual Abstract)

Background: In the Korean population, gene frequencies of Human Neutrophil Alloantigen-1 (HNA-1), HNA-2, HNA-4, and HNA-5 were determined using serological and genotyping methods. However, no study assessing the gene frequencies of HNA-3 among the Korean population has been conducted. The aims of this study were to report the gene frequencies of HNA-3, to estimate the risk of HNA-3a (or HNA-3b) alloimmunization, and to secure donors of granulocytes for anti-HNA-3 antibodies among the Korean population.
Methods: Genotyping of HNA-3a and HNA-3b genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers.
Results: The observed frequencies of HNA-3a and HNA-3b were 0.695 and 0.305, respectively, among the Korean population. The HNA-3a gene frequency of Koreans was significantly lower than those of American Whites and Germans (P＜0.05). The risk of HNA-3a and HNA-3b alloimmunization due to pregnancy (transfusion) was 0.065 (0.084) and 0.147 (0.250), respectively, among the Korean population. The risk of HNA-3a alloimmunization was significantly higher in the Korean population than in the German and American White populations (P＜0.05).
Conclusion: The gene frequencies of HNA-3 and the risk of HNA-3a (HNA-3b) alloimmunization due to pregnancy or transfusion among the Korean population were determined. We also identified individuals who were HNA-3a/3a or HNA-3b/3b for the granulocyte panel which could be used for anti-HNA antibody identification.

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