Poster Session : PS 0411 ; Infectious Disease ; High Prevalence of Hepatitis B Virus Infection Among Indonesian Migrant Workers from Lombok Island, Indonesia and Their Complete Genome Analysis = Poster Session : PS 0411 ; Infectious Disease ; High Prevalence of Hepatitis B Virus Infection Among Indonesian Migrant Workers from Lombok Island, Indonesia and Their Complete Genome Analysis

다국어 초록 (Multilingual Abstract)

Background: Due to the high endemicity of hepatitis B in Lombok population, it is important to determine its prevalence among migrant workers (MWs), since Lombok contributes the most from Indonesia abroad. Hepatitis B virus (HBV) (sub)genotypes have geographic specifi c distribution. We have reported HBV strains from Indonesian MWs based on part of envelope region and several strains could not clearly be determined their subgenotypes. In this study we analyse the whole genome sequences of those strains. Methods: Sera were collected from Indonesian MWs, to be screened for HBsAg by Enzyme Immunoassay. Those with HBsAg positive were continued with HBV DNA detection and (sub)genotype/subtype determination based on envelope region and, for several strains, on whole genome sequences. Results: Seventeen point two percent (15/87) sera from the MWs who will go to their destination countries and 12.2% (15/124) of those come back to Indonesia were HBsAg positive. All of 30 HBsAg positive samples showed hepatitis B viremia by part of envelope gene amplifi cation. Six of HBV-B genotype and one HBV-C strains were whole genome sequenced. All together showed that the most isolates (96.2%, 25/26) belonged to HBV B3/adw2 and 3.8% was HBV C8/adrq+. The whole genome phylogenetic analysis of our strains supports a recent argument that subgenotypes B5, B7, B8, B9 could be considered as a quasi-subgenotype of B3, which is most commonly found in West Indonesia, including in Lombok. Conclusions: High prevalent of HBsAg carriers in the Indonesian MWs from Lombok were found, with the predominant of HBV B3/adw2. A tight screening of HBV to the MWs should be applied, in order to limit its transmission abroad. This study also confi rmed a recent fi nding that HBV-B subgenotypes need to be reclassifi ed.