계태아 발생시 TGF-ß3가 구개판 내측돌기상피의 상피간엽변환 및 상피성장인자수용체 발현에 미치는 영향

다국어 초록 (Multilingual Abstract)

Cleft lip and /or palate is the congenital orofacial malformation most commonly occurred in humans. The disease is multifactorial and is probably caused by genetic and/or environmental factors. So, there are many problems in research concerning etiology and in treatment of the disease. Even the most practiced and sophisticated methods of surgical repair are necessarily followed by scar contraction and fibrosis, which result in skeletal defects, dental abnormalities, cosmetic disfigurement, and speech impairment. As a result, Fetal surgery can be considered but practiced rarely when the deformity is not fatal to life. And treatment of cleft palate is performed in the form of medicine projection into uterus in animal experiments.
Many studies show that growth factor and its receptor emerge from the developing palate; and the epidermal growth factor receptors have a important role in craniofacial development and in palatal fu5ion. The palatal morphogenesis of the avine is different from the mammal s; it takes the form of physiologic cleft palate. Recently, cleft palate fusion experiment was performed when the avine were in the period of palate formation through the exogenous TGF-ß3 addition, and it showed that the exogenous TGF-P3 makes fusion of divided palate through certain process when cleft palate is occurred in palatal formation. In this study, I had the confirmation of the fusion of cleft palate through the addition of TGF-j3 in case of chicken embryo, and observed the effect of TGF- ß in EGF receptor distribution. And the following is the results of this study.
1. In case of the TGF-Pl and p3 addition group, there was the decrease of EGFR(Epidermal Growth Factor Receptor) immunoreactivity in mesenchymal cells beneath the medial edge epithelium and also in epithelial mesenchymal interface which is between medial edge epithelium and nasal septum in 72 hours.
2. The immunoreactivity of the control group resembles that of normal chicken embryo palate in development.
3. In the view through fluorescence confocal microscopy, there was confluence in TGF-P3 addition group. This shows that the confluence induced by exogenous TGF-P3 is related to EGFR expression in palate of chicken embryo, which is a physiologic cleft palate model.