Eight strains of Drosophila melanogaster belonging to different localities of Japan were subject to intraspecific mitochondrial DNA(mtDNA) variation studies using 11 restriction endonucleases. Length variation was detected in HindⅢ restriction endon...
Eight strains of Drosophila melanogaster belonging to different localities of Japan were subject to intraspecific mitochondrial DNA(mtDNA) variation studies using 11 restriction endonucleases. Length variation was detected in HindⅢ restriction endonuclease fragments and site variation was revealed in HpaⅡ,HaeⅢ and ScaⅠrestriction endonucleases fragments. Japanese strains here studies share the same mtDNA genotypes as Korean strain previously reported, but one strain collected in Ogasaware Island showed a distinct genotype. Average substitution rate of Japanese strains(2.2%) was found to be about seven times higher than that of Korean strains(0.34%),because Ogasawara Island strain seem to be completely isolated without migration. A possible explanation of this result was that they have been subject to a recent diversification of a monomorphic maternal lineage. MtDNA divergence between Korean and Japanese population is low(Gst=8.3%). From this results, we confirmed that no evidence of mtDNA divergence between two countries was obtained.