PFGE를 이용한 Brucella abortus의 유전형별 = Genotyping of Brucella abortus by PFGE

다국어 초록 (Multilingual Abstract)

The process of subtyping is important epidemiologically for recognizing outbreaks of infection. Pulsedfield gel electrophoresis (PFGE) is considered the “gold standard” of molecular typing methods and a method of seperating large DNA molecules. In our study, Electrophoresis of DNA digested with the
restriction endonuclease Xba I, Xho I and Smi I produced fragment profiles. Digestion of genomic DNA with Xba I and Xho I provided results that not distinguished among field strains of Brucella abortus which tested in study. But Smi I restriction enzyme resulted in two PFGE pattern consisting of 13-15 bands that ranged in size from 33 to 668 standard marker. Analysing DNA fingeprinting software, cluster analysis showed 93.75% similar in two PFGE pattern.

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