사람의 체온을 이용한 먹는물 판별 방법 연구

다국어 초록 (Multilingual Abstract)

Aiming to figure out a fast and no-energy method to detect coliforms and Salmonella spp. in drinking water or recreational water, we first assessed the usability of an enzymatic method and an immunochromatography test which identify the existence of coliforms and Salmonella spp. in random water. In previous study, we had developed the enzymatic method to detect coliforms in water by using only human body surface temperature instead of using the incubator needed electricity. To detect Salmonella spp. additionally, we used commercially available immunochromatography test kits for Salmonella spp. After incubating water samples for 24 hours to detect coliforms by using body surface temperature, we conducted the immunochromatography test with the culture samples. We found that coliforms and Salmonellas spp. could be detected simultaneously in the culture using body surface temperature only. We concluded that using human body surface temperature, it is possible to test drinking water within 24 hours, without any energy involved.

참고문헌 (Reference)

1 이규철, "엔테로코커스 검출을 위한 막여과법과 효소발색법의 비교" 한국엔터테인먼트산업학회 5 (5): 193-198, 2011

2 World Health Organization, "Water Quality and Health Strategy 2013-2020"

3 I. George, "Use of enzymatic methods for rapid enumeration of coliforms in freshwaters" 8 : 404-413, 2000

4 M. Dominguez, "The MIT D-lab electricity-free PortaTherm™ incubator for remote testing with the QuantiFERON®-TB Gold In-Tube assay" 14 : 1468-1474, 2010

5 E.F. Schumacher, "Small is beautiful: Economics as if people really mattered" Abacus 1973

6 O. Traoré, "Prevalence and diversity of Salmonella enterica in water, fish and lettuce in Ouagadougou, Burkina Faso" 15 : 151-, 2015

7 World Health Organization, "Guidelines for drinking-water quality: incorporating first addendum"

8 World Health Organization, "Fact sheet N°330"

9 S. Nam, "Detection of coliforms in drinking water using skin patches: a rapid, reliable method that does not require an external energy source" 90 (90): 283-287, 2014

10 United States Environmental Protection Agency, "Basic Information about Pathogens and Indicators in Drinking Water"

11 H.M. Murphy, "Appropriate technology – A comprehensive approach for water and sanitation in the developing world" 31 : 158-167, 2009

12 Appropedia, "Appropriate technology"

13 O. Savichtcheva, "Alternative indicators of fecal pollution: relations with pathogens and conventional indicators, current methodologies for direct pathogen monitoring and future application perspectives" 40 : 2463-2476, 2006