白鼠의 中大腦動脈의 폐쇄와 재관류에 대한 실험모양인 頭蓋腔外 접근법과 經頭蓋腔 접근법에 대한 비교연구 : Extracranial and Transcranial Methods = Reperfusion Models of Rat Middle Cerebral Artery Occlusion

다국어 초록 (Multilingual Abstract)

The author described a comparative study of 2 different expenemental methods of reperfusion models in rat ; 1) transcranial method(TC) and 2) extracranial method(EC). The transcranial method was performed by a direct approach to the middle cerebral artery(MCA) after temporozygomatic craniotomy in which a wire was hooked on the proximal portion of middle cerebral artery(MCA) and pulled it to make occlusion of MCA. For the extracranial method a nylon surgical thread was inserted intraluminally along the internal carotid artery(ICA) to reach the anterior communicating artery. Recirculation of blood was accomplished by removal of the hook or of the thread after 4 hours occlusion of MCA. Two different models were subdivided into 4 chronological goups pre-reperfusion, 30 minutes, 2 hours, and 48 hours after reperfusion. The differences were evaluated with morphometric analysis after neutral red(NR) or tetrazolium salt(TTC) staining.
From the evaluation of initial ischemic area, the extracranial method showed a larger infarct volume as compared to the transcranial method(p=0.013). Mean value(SD) of infarcted area of EC and TC were 28.7% (4.4%) and 14.4% (8.1%) respectively. Recovery from the initial ischemia after reperfusion was gradually achieved in both of two groups(p<0.05). The restoration of blood flow after reperfusion was more rapid in EC group and only 2 hours reperfusion was enough to show no difference between two methods. In TC group followed by 48 hours' reperfusion, 40% of animals resulted in gross infarction of MCA temtory as noted with TTC stain, most probably due to reperfusion failure. EC group showed only one such a case(12.5 %). But this difference was not in the range of significance statistically. From a technical point of view, EC was more simple, needed less time since a craniotomy was not performed. and required no manipulation of the brain or any intracranial vessels. From these results the author concluded that the extracranial method is a more reliable and technically more manageable one to be used as a reperfusion model for investigating the focal cerebral ischemia.