다발성 경화증 동물 모델에서 중간엽줄기세포와 미코페놀레이트모페틸의 병용치료 효과

다국어 초록 (Multilingual Abstract)

Multiple sclerosis (MS) is a chronic autoimmune disease that affects the central nervous system. Immunosuppressants, such as mycophenolate mofetil (MMF), have a reported relative safety profile as treatments of MS. Mesenchymal stem cells (MSCs), which have immunomodulatory and regenerative properties, have also been proposed as a potential cell therapy for MS. MSCs have been shown to inhibit the activation and proliferation of immune cells, including T and B cells, which play a key role in the pathogenesis of MS, and have been shown to promote the regeneration of damaged nerve cells. In this study, we examined whether combined administration of MMF and MSCs in Experimental autoimmune encephalomyelitis (EAE) mice, a widely used animal model * A thesis for the degree of Master in August 2024. for MS research, would be more effective than each alone. Our data showed that compared to each therapy, the combination therapy was more effective in reducing the disease progression in EAE mice and in reducing the cytokine expression in the spinal cord, spleen, and lymph nodes. In summary, our results indicate that combination therapy of MMF and MSCs might be effective in ameliorating therapy of MMF and MSCs might be effective in ameliorating the symptoms in EAE mice, which provides information of how to treat MS patients. Key words: Multiple sclerosis, Mesenchymal stem cell, Mycophenolate mofetil, Experimental autoimmune encephalomyelitis mice

목차 (Table of Contents)

• I. 서론 1
• II. 실험 재료 및 방법 4
• 1. 중간엽 줄기세포 배양 4
• 2. 별아교세포 배양 4
• 3. 미세아교세포 배양 5
• 4. 면역억제제 5
• 5. 실험동물 6
• 6. 면역조직병리학(Immunohistopathology) 7
• 7. 실시간 역전사 중합효소 연쇄반응법 (Quantitative real-time polymerase chain reaction, RT-PCR) 7
• 8. 효소면역 측정법 (Enzyme-linked immunosorbent assay, ELISA) 11
• 9. 세포 증식 측정법 (Celltiter glo) 11
• 10. 통계 처리 12
• III. 실험 결과 13
• 1. 병용투여 효과 13
• 1.1. 중간엽 줄기세포와 mycophenolate mofetil의 병용투여 13
• 1.2. 척수 내 병용투여 효과 15
• 1.3. 전신면역 측면 병용투여 효과 18
• 2. 신경교세포에 대한 면역억제제의 영향 21
• 2.1. 별아교세포의 생존율 및 사이토카인 발현 21
• 2.2. 미세아교세포의 생존율 및 사이토카인 발현 23
• 3. 활성화된 신경교세포의 IL-6에 대한 중간엽 줄기세포의 역할 25
• IV. 고찰 27
• V. 결론 29
• 참고문헌 30