Effects of compound traditional Astragalus and Salvia Miltiorrhiza extract on acute and chronic hepatic injury

다국어 초록 (Multilingual Abstract)

Previous reports showed that Compound Astragalus and Salvia miltiorrhiza extract (CASE), which was mainly composed of astragalosides, astragalus polysaccharide and salvianolic acids, inhibited hepatic fibrosis by mediating transforming growth factor-β (TGF-β)/Smad signaling. Our aim was to examine the effects of CASE on D-galactosamine (D-GalN) treated liver injury in mice and carbon tetrachloride (CCl4)-induced liver fibrosis in rats. CASE was administered to mice with D-GalN-induced liver injury and to rats with CCl4-induced liver fibrosis, respectively. Liver injury was routinely evaluated by relative liver weight, serum levels of ALT, AST, hyaluronic acid (HA), hepatic malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, hydroxyproline (HYP) and histopathologic changes. Treatment of mice with CASE (60, 120, and 240 mg/kg, ig) significantly lowered ALT, relative liver weight, and MDA levels when compared with D-GalN treated mice. CASE (120, 240 mg/kg) significantly lowered ALT, AST, HA, HYP, and MDA levels against CCl4 treated rats. Decreased SOD level was reversed with CASE treatment. Upon histopathological examination, CASE treatment had significantly inhibitory effect on the progression of hepatic fibrosis in rats. These results indicate that CASE might be effective in treatment and prevention of acute and chronic hepatic injury due to its antioxidant activity.

참고문헌 (Reference)

1 Karadeniz A, "The effects of Panax ginseng and Spirulina platensis on hepatotoxicity induced by cadmium in rats" 72 : 231-235, 2009

2 Li C, "The collagenolytic effects of the traditional Chinese medicine preparation, Han- Dan-Gan-Le, contribute to reversal of chemical- induced liver fibrosis in rats" 72 : 1563-1571, 2003

3 Liu J, "Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells" 153 : 85-93, 2000

4 Iredale JP, "Mechanisms of spontaneous resolution of rat liver fibrosis. Hepatic stelllate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors" 102 : 538-549, 1998

5 Lin YL, "In vitro protective effects of salvianolic acid B on primary hepatocytes and hepatic stellate cells" 105 : 215-222, 2006

6 Kang H, "Immunomodulatory effect of Astragali Radix extract on murine TH1/TH2 cell lineage development" 27 : 1946-1950, 2004

7 Shyu MH, "Hsian-tsao (Mesona procumbens Heml.) prevents against rat liver fibrosis induced by CCl4 via inhibition of hepatic stellate cells activation" 46 : 3707-3713, 2008

8 Stachlewitz RF, "Glycine and uridine prevent Germolec D, Thurman RG. Glycine and uridine prevent D-galactosamine hepatotoxicity in the rat: role of Kupffer cells" 29 : 737-, 1999

9 Huang XZ, "Experimental researc of the various doses treatment groups of Salvia miltiorrhiza to prevent liver fibrosis induced with CCl4. Integr" 11 : 213-215, 2001

10 Tsukamoto H, "Experimental models of hepatic fibrosis: a review" 10 : 56-65, 1990

11 Zhang XX, "Effect of total polysaccharide of Astragalus on proliferation and collagen production of HSC-T6 cells" 8 : 645-647, 2003

12 Wu Q, "Effect of astragalosides on proliferation and collagen production of hepatic stellate cells in vitro" 19 : 892-895, 2003

13 Liu X, "Compound Astragalus and Salvia miltiorrhiza extract inhibits cell invasion by modulating transforming growth factor-b/Smad in HepG2 cell" 25 : 420-426, 2010

14 Yang Y, "Compound Astragalus and Salvia miltiorrhiza Extract exerts anti-fibrosis by mediating TGF-beta/Smad signaling in myofibroblasts" 118 : 264-270, 2008

15 Roxas M, "Colds and influenza: a review of diagnosis and conventional, botanical, and nutritional considerations" 12 : 25-48, 2007

16 Hu S, "Antitumor effects of Chi Chi-Shen extract from Salvia miltiorrhiza and Paeoniae radix on human hepatocellular carcinoma cells" 28 : 1215-1223, 2007