한국 자생란에서 난 균근균의 분리와 유묘난에 접종 = Isolations of the Orchid mycorrhizal Fungi from the Roots of the Korean native Orchids and Inoculations of the Isolates to Four different Orchids

국문 초록 (Abstract)

한국의 춘란 자생지의 춘란(Cymbidium goeringii) 뿌리에서 공생하는 10개의 균근균을 분리하였다. 분리된 난 균근균을 PDA에서 배양한 결과 균총은 다양한 색깔을 나타내었으며, 생장 속도도 달랐다. 분리된 난 균근균을 현미경으로 관찰한 결과 Rhizoctonia repens 또는 R. endophytica var endophytica로 동정되었다. R. repens는 자생란인 춘란의 뿌리에서 많이 분리되었으며, R. endophytica var endophytica는 상업적으로 재배되는 외국산의 난 뿌리에서 분리되었다. 분리된 균들 중에 R. repens와 유사한 5개의 분리균을 oatmeal agar에 접종하고 배양한 후 여러종의 난 유묘를 이식하여 난의 생장을 관찰하였다. 그 결과 난의 생장은 분리 균에 따라서 다양하였으나 대부분의 난은 생장촉진 반응을 나타내서, 분리균과 각각의 난과의 공생 관계를 관찰할 수 있었다.

다국어 초록 (Multilingual Abstract)

Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophytica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorphic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observed by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different from different isolates due to the genetic variations.