05 포스터 발표 : 환경 화학 분야(PC) ; LC-MS/MS를 이용한 현미와 볏짚에서의 Benzobicyclon과 대사체 잔류 분석 = Analysis of Benzobicyclon and its Metabolite in Brown Rice and Rice Straw after Field Application Using Liquid Chromatography-tandem Mass Spectrometry

다국어 초록 (Multilingual Abstract)

This study was carried out to develop an extraction as well as an analytical method for detecting benzobicyclon and its amino-substituted metabolite (1315P-570) in brown rice and rice straw using liquid chromatography-tandem mass spectrometry (LC/MS/MS) in positive ionmode with multiple reaction monitoring (MRM). The parent as well as the metabolite in rice and rice straw were extracted and analyzed under the same conditions. The matrix-matched calibrations showed good linearity with determination coefficients ( R2) of > 0.994. Recoveries at two different spiking levels were satisfactory and ranged between 75.4.118.9% with relative standard deviations (RSDs) < 13% for both analytes. Under storage conditions (.20℃), the analyte and its metabolite were stable for up to 92 days. The limits of quantitation (LOQs) were lower than the maximum residue level (MRL) set by the Ministry of Food and Drug Safety for brown rice (0.1 mg/kg). Field trials with recommended or double the recommended dose revealed that the herbicide could safely be applied to rice and rice straw, as no residues were detected in the harvested samples. The sensitivity of the developed method was sufficient to ensure reliable determination of benzobicyclon and its metabolite in rice grain and rice straw.