Calmodulin유전자의 Sense와 Antisense구조를 가진 감자 Transgenic Plant의 기내 생장과 발육 = Differences in in vitro Growth and Development of Transgenic Potato Plants Carrying Sense and Transgenic Potato Plants Carrying Sense and Antisense Constructs of Calmodulin Gene

다국어 초록 (Multilingual Abstract)

Single-node stem segments of transgenic potaot plants(solanum tuberosun L. var. FL1607) carrying patatin promotor fused to sense or antisense construct of PCM1 were used to investigate in vitro growth and development of them as influenced by different sucrose concentrations in thed가 or light(16th/day) at 16℃.
During 15days after treatments, a tuber formed at the etiolated shoot sprouted from the auxiliary bud of single-node stem segments only in the dark condition. Tuberization was increased by increasing the sucrose concentration in the media. The stem segments grown in the media without sucrose, which were cultured under the dark or light conditions, showed little growth and development.
Tuber induction was delayed in the sense plant as compared to the control plant. There was little difference between control and antisense plants in tuber formation. In the light condition, the sprouting shoot from the bud showed upright growth in the low sucrose concentration(2%) and horizontal growth with basal swelling in the high sucrose concentration(4∼8%).
The higher the sucrose concentration in the media, the more reduction of shoot growth was observed in all plants. During this time, the shoot growth was inhibited in the antisense plant and was increased in the sense plant as compared to the control plant. PCM1 gene expressions of the control and sense plants incubated under the light condition were a little higher than those of these plants incubated under the dark condition. However, no difference was found in this gene expression between the control and sense plants cultured under the same conditions.