Expression of CIP1 gene induced under cadmium stress in Candida sp.

다국어 초록 (Multilingual Abstract)

Cadmium is a toxic heayy metal with no known bloioglcal activity Differential display of mRNA was employed to isolate cDNA corresponding to the transcript that is induced in cadmium-treated Candida sp in this report we describe the molecular characterization of CIP1 gene, which was shown to be rapidly induced after cadmium treatment. Northern blot analysis showed that CIP1 transcript was not present in normal cell, but accumulated at higher levels in cadmium-treated cells Treatment of other heavy metals such as cupper, mercury, lead, zinc, or heat shock had no effect on the expression of CIP1 gene Sequence analysis of CIP1 revealed that it encodes a 32 kDa hydrophobic protein that contains a putative transmembrane domain The deduced amino acid sequence of CIP1 showed a little homology with isoflayone reductase of plant From the promoter sequence analysis we also identified a sequence similar to pas, a cadmium-responsive element of ParA gene in tobacco Our results suggest that Candida CIP1 may play a crucial role in the establishment of specific cellular response to stress evolved by the cadmium treatment

목차 (Table of Contents)

• INTROCUCTION
• MATERIALS AND METHODS
• RESULTS AND DISCUSSION
• ACKNOWLEDGEMENTS
• REFERENCES
• FIGURE LEGENDS