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      KCI등재 SCOPUS

      개양귀비(Papaver rhoeas L.)로부터 분리한 세포외 다당류 생성 유산균 Weissella cibaria PE17의 특성 = Isolation and characterization of exopolysaccharide-producing Weissella cibaria PE17 from corn poppy (Papaver rhoeas L.)

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      https://www.riss.kr/link?id=A109044412

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      다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

      Exopolysaccharide (EPS)-producing lactic acid bacteria strain PE17 was isolated from corn poppy (Papaver rhoeas L.) to investigate its EPS production ability and probiotic characteristics. PE17 was identified as Weissella cibaria using 16S rRNA gene sequencing. Weissella cibaria PE17 produced 4.66±0.01 g/L of purified EPS; the purified EPS consisted of only glucose. Additionally, W. cibaria PE17 survived in an intestinal environment. The isolate was confirmed to be safe for use, as it did not produce hemolytic and harmful metabolites and enzymes. Treatment of the cell-free supernatant of the isolate with different concentrations of Cutibacterium acnes and Staphylococcus aureus inhibited the growth and the biofilm formation of both strains in a dose-dependent manner. Wessella cibaria PE17 may be useful for manufacturing fermented food, cosmetics, and probiotics.
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      Exopolysaccharide (EPS)-producing lactic acid bacteria strain PE17 was isolated from corn poppy (Papaver rhoeas L.) to investigate its EPS production ability and probiotic characteristics. PE17 was identified as Weissella cibaria using 16S rRNA gene s...

      Exopolysaccharide (EPS)-producing lactic acid bacteria strain PE17 was isolated from corn poppy (Papaver rhoeas L.) to investigate its EPS production ability and probiotic characteristics. PE17 was identified as Weissella cibaria using 16S rRNA gene sequencing. Weissella cibaria PE17 produced 4.66±0.01 g/L of purified EPS; the purified EPS consisted of only glucose. Additionally, W. cibaria PE17 survived in an intestinal environment. The isolate was confirmed to be safe for use, as it did not produce hemolytic and harmful metabolites and enzymes. Treatment of the cell-free supernatant of the isolate with different concentrations of Cutibacterium acnes and Staphylococcus aureus inhibited the growth and the biofilm formation of both strains in a dose-dependent manner. Wessella cibaria PE17 may be useful for manufacturing fermented food, cosmetics, and probiotics.

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      참고문헌 (Reference)

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      3 Sangy S, "The effects of gavage extract of Papaver rhoeas L. on blood pressure of MIA induced osteoarthritis and its interaction with nitrergic system in male rat" 2 : 458-466, 2020

      4 Ahmed S, "The Weissella genus : Clinically treatable bacteria with antimicrobial/probiotic effects on inflammation and cancer" 10 : 2427-, 2022

      5 Abarquero D, "Study of exopolysaccharides from lactic acid bacteria and their industrial applications : a review" 57 : 16-26, 2022

      6 Pumriw S, "Screening of probiotic lactic acid bacteria isolated from fermented Pak-Sian for use as a starter culture" 78 : 2695-2707, 2021

      7 Sharma K, "Purification and characterization of novel exopolysaccharides produced from Lactobacillus paraplantarum KM1 isolated from human milk and its cytotoxicity" 18 : 1-10, 2020

      8 Adesulu-Dahunsi AT, "Production, characterization and in vitro antioxidant activities of exopolysaccharide from Weissella cibaria GA44" 87 : 432-442, 2018

      9 Rabetafika HN, "Probiotics as antibiotic alternatives for human and animal applications" 3 : 561-581, 2023

      10 Dimofte A, "Probiotic properties of Weissella confusa PP29 on Hibiscus sabdariffa L" 8 : 553-, 2022

      1 Kim MJ, "Weissella cibaria CMU exerts an anti-inflammatory effect by inhibiting Aggregatibacter actinomycetemcomitans-induced NF-κB activation in macrophages" 22 : 4143-4150, 2020

      2 Lei F, "The macleaya cordata symbiont : revealing the effects of plant niches and alkaloids on the bacterial community" 12 : 681210-, 2021

      3 Sangy S, "The effects of gavage extract of Papaver rhoeas L. on blood pressure of MIA induced osteoarthritis and its interaction with nitrergic system in male rat" 2 : 458-466, 2020

      4 Ahmed S, "The Weissella genus : Clinically treatable bacteria with antimicrobial/probiotic effects on inflammation and cancer" 10 : 2427-, 2022

      5 Abarquero D, "Study of exopolysaccharides from lactic acid bacteria and their industrial applications : a review" 57 : 16-26, 2022

      6 Pumriw S, "Screening of probiotic lactic acid bacteria isolated from fermented Pak-Sian for use as a starter culture" 78 : 2695-2707, 2021

      7 Sharma K, "Purification and characterization of novel exopolysaccharides produced from Lactobacillus paraplantarum KM1 isolated from human milk and its cytotoxicity" 18 : 1-10, 2020

      8 Adesulu-Dahunsi AT, "Production, characterization and in vitro antioxidant activities of exopolysaccharide from Weissella cibaria GA44" 87 : 432-442, 2018

      9 Rabetafika HN, "Probiotics as antibiotic alternatives for human and animal applications" 3 : 561-581, 2023

      10 Dimofte A, "Probiotic properties of Weissella confusa PP29 on Hibiscus sabdariffa L" 8 : 553-, 2022

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      15 Hoxha R, "New lactic acid bacterial strains from traditional fermented foods-bioprotective and probiotic potential" 58 : 252-269, 2023

      16 Tsigkrimani M, "Microbial ecology of sheep milk, artisanal feta, and kefalograviera cheeses. Part II:Technological, safety, and probiotic attributes of lactic acid bacteria isolates" 11 : 459-, 2022

      17 Tamura K, "MEGA11 : molecular evolutionary genetics analysis version 11" 38 : 3022-3027, 2021

      18 Ibrahim SA, "Lactic acid bacteria as antimicrobial agents : Food safety and microbial food spoilage prevention" 10 : 3131-, 2021

      19 Franco W, "Isolation of exopolysaccharide-producing yeast and lactic acid bacteria from quinoa(Chenopodium quinoa)sourdough fermentation" 9 : 337-, 2020

      20 Lafta AA, "Isolation of alkaloids from Papaver rhoeas(Papaveraceae)wildly grown in Iraq" 30 : 78-85, 2021

      21 Tóth P, "Investigation and characterisation of new ecofriendly cosmetic ingredients based on probiotic bacteria ferment filtrates in combination with alginite mineral" 10 : 2672-, 2022

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      24 Kang MS, "In vitro preventive effect and mechanism of action of Weissella cibaria CMU against Streptococcus mutans biofilm formation and periodontal pathogens" 11 : 962-, 2023

      25 Daliri EB-M, "In vitro and in vivo cholesterol reducing ability and safety of probiotic candidates isolated from Korean fermented soya beans" 14 : 87-98, 2022

      26 Gezginç Y, "Health promoting benefits of postbiotics produced by lactic acid bacteria : Exopolysaccharide" 31 : 61-70, 2022

      27 Araya M, "Guidelines for the evaluation of probiotics in food. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food" World Health Organization-Food and Agricultural Organization 1-11, 2002

      28 Chen T, "Functional probiotics of lactic acid bacteria from Hu sheep milk" 20 : 1-12, 2020

      29 Buksa K, "Extraction, purification and characterisation of exopolysaccharides produced by newly isolated lactic acid bacteria strains and the examination of their influence on resistant starch formation" 362 : 130221-, 2021

      30 Xu Y, "Exopolysaccharides produced by lactic acid bacteria and Bifidobacteria : Structures, physiochemical functions and applications in the food industry" 94 : 475-499, 2019

      31 Jurášková D, "Exopolysaccharides produced by lactic acid bacteria : From biosynthesis to health-promoting properties" 11 : 156-, 2022

      32 Sørensen HM, "Exopolysaccharides of lactic acid bacteria : Production, purification and health benefits towards functional food" 14 : 2938-, 2022

      33 Nguyen P, "Exopolysaccharide production by lactic acid bacteria : The manipulation of environmental stresses for industrial applications" 6 : 451-, 2020

      34 Saputra S, "Exopolysaccharide production and amylase activity of lactic acid bacteria strains" 2606 : 020013-, 2023

      35 Kim H, "Ethyl acetate fractions of Papaver rhoeas L. and Papaver nudicaule L. exert antioxidant and anti-inflammatory activities" 10 : 1895-, 2021

      36 Punyauppa-path S, "Diversity of fermentative yeasts with probiotic potential isolated from Thai fermented food products" 8 : 575-594, 2022

      37 Ruiz Rodríguez LG, "Diversity and functional properties of lactic acid bacteria isolated from wild fruits and flowers present in Northern Argentina" 10 : 1091-, 2019

      38 Marsoul A, "Determination of polyphenol contents in Papaver rhoeas L. flowers extracts (soxhlet, maceration), antioxidant and antibacterial evaluation" 31 : S183-S189, 2020

      39 Mayslich C, "Cutibacterium acnes as an opportunistic pathogen : An update of its virulence-associated factors" 9 : 303-, 2021

      40 Grauso L, "Corn poppy, Papaver rhoeas L.: a critical review of its botany, phytochemistry and pharmacology" 20 : 227-248, 2021

      41 Daba GM, "Contributions of exopolysaccharides from lactic acid bacteria as biotechnological tools in food, pharmaceutical, and medical applications" 173 : 79-89, 2021

      42 Werning ML, "Biological functions of exopolysaccharides from lactic acid bacteria and their potential benefits for humans and farmed animals" 11 : 1284-, 2022

      43 Rwubuzizi R, "Beneficial, safety, and antioxidant properties of lactic acid bacteria: A next step in their evaluation as potential probiotics" 9 : e15610-, 2023

      44 Hernández-González JC, "Bacteriocins from lactic acid bacteria. A powerful alternative as antimicrobials, probiotics, and immunomodulators in veterinary medicine" 11 : 979-, 2021

      45 Kim JH, "Antibiofilm activity of crude bacteriocin JM01 produced by Pediococcus acidilactici against methicillin-resistant Staphylococcus aureus(MRSA)" 58 : 2580-2589, 2023

      46 Cristofori F, "Anti-inflammatory and immunomodulatory effects of probiotics in gut inflammation : a door to the body" 12 : 578386-, 2021

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