한국재래닭의 Uncoupling protein 유전자 Exon 3에서의 +1316T/T 유전자형이 산란율에 미치는 효과 분석 = The +1316 T/T Genotype in the Exon 3 of Uncoupling Protein Gene is Associated with Daily Percent Lay in Korean Native Chicken

다국어 초록 (Multilingual Abstract)

Uncoupling protein(UCP) is expressed exclusively in brown adipose tissue(BAT). It is known to uncouple phosphorylation from oxidation and hence to be involved in energy metabolism and heat production, especially under cold exposure. In the present study, we identified single nucleotide polymorphism(SNP) in exon 3 of avUCP gene in Korean native chicken(KNC) population. It was detected a SNP T+1316C in exon 3 of avUCP gene by sequence analysis in KNC population. For PCR-RFLP analysis of the SNP T+1316C, used by Afl III restriction enzyme. The result of PCR-RFLP analysis showed that allele T has two fragments of 255 bp and 86 bp, and allele C has only one fragment of 341 bp. The genotype frequencies were TT type, 0.7875; TC type, 0.1875 and CC type, 0.025; and, the frequencies of allele T and C were 0.881 and 0.119, respectively in KNC population. Next study was conducted to investigate the effect of the SNP in avUCP gene on economic traits in the KNC population. The TT genotype had a significant higher daily percent lay(84.61) than CC genotype(p<0.05) in KNC population. This study may be useful for genetic studies of avUCP gene and selection on daily percent lay of KNC.

국문 초록 (Abstract)

Uncoupling protein(UCP)은 갈색 지방세포에서 특이적으로 발현하고 있으며 복잡한 세포의 열 생산 작용에 관여한다고 알려져 있다. 본 연구는 한국 재래닭 집단의 UCP 유전자내에 존재하는 SNP를 검출하였다. 한국 재래닭 집단의 UCP 유전자 exon 3지역의 염기서열 분석 결과 1316 bp에서 T염기가 C염기로 치환되어짐을 확인하였다. T+ 1316C 지역의 PCR-RFLP 분석을 위해 제한효소 Afl Ⅲ를 사용하였다. 한국 재래닭 집단내 유전자형 빈도는 TT가 0.7875, TC가 0.1875 그리고 CC가 0.025로 검출되었으며 대립유전자의 빈도는 T가 0.881 그리고 C가 0.119로 나타났다. 또한 검출된 SNP가 경제형질에 미치는 영향을 분석한 결과 한국 재래닭 집단의 T/T 유전자형과 C/C 유전자형에서 일당 산란율에서 통계적으로 유의한 차이가 있음을 확인하였다. 본 연구의 결과는 향후 더 많은 UCP 유전자와 관련된 연구와 한국 재래닭의 육종 전략에 도움이 될 것으로 사료된다.

참고문헌 (Reference)

1 "comparison with rat gene and assignment to the long arm of chromosome 4" 255-264,

2 "cellular and molecular aspects of avian muscle non-shivering thermogenesis" 151-165,

3 "an coupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue" 79-82, 1997

4 "a novel gene linked to obesity and hyperinsulinemia" 269-272,

5 "a new member of the mitochondrial carrier family with tissue-specific expression" 39-42, 1997

6 "Uusitupa M 2000 Synergistic effect of polymorphisms of uncoupling protein 1 and beta 3-adrenergic receptor genes on long-term body weight change in Finnish type 2 diabetic and non-diabetic control subjects" 514-519, 2000

7 "Uusitupa M 1998 Additive effects of the mutations in the beta 3-adrenergic receptor and uncoupling protein-1 genes on weight loss and weight maintenance in Finnish women" j clin endocri (j clin endocri): 4246-4250, 1998

8 "Tartaglia LA 1997 Cloning and characterization of an uncoupling protein homolog" gime (gime): 900-46 906, 1997

9 "Strosberg AD 2000 Association of sets of alleles of genes encoding beta 3-adrenoreceptor uncoupling protein 1 and lipoprotein lipase with increased risk of metabolic complications in obesity" 93-100, 2000

10 "Ricquier D 2001 Homologues of the uncoupling protein from brown adipose tissue" 107-1 119, 2001

11 "Ricquier D 2001 An coupling protein homologue putatively involved in facultative thermogenesis in birds" 161-179, 2001

12 "Pierre B 2004 The common -866G/A polymorphism in the promoter of uncoupling protein 2 is associated with increased carbohydrate and decreased lipid oxidation in juvenile obesity" 53 : 235-239, 2004

13 "Laakso M 1998 Different regulation of free fatty acid levels and glucose oxidation by the Trp64Arg polymorphism of the beta 3-adrenergic receptor gene and the promoter variant of the uncoupling protein 1 gene in familial combined hyperlipidemia" 1397-47 1402,

14 "Kondo M 1998 Synergic effect of polymorphisms in uncoupling protein 1 and beta 3-adrenergic receptor genes on weight loss in obese Japanese" -41, 1998

15 "Kobayashi K 1999 Phenotypic characterization of the beta 3-adrenergic receptor mutation and the uncoupling protein 1 polymorphism in Japanese men" 36-48 40, 1999

16 "Jezek PJ 1996 On the mechanism of fatty acid-induced proton transport by mitochondrial uncoupling protein" 2615-2620, 1996

17 "Froguel P 1996 Additive effect of A→G variant of the uncoupling protein gene and the Trp64Arg mutation of the beta 3-adrenergic receptor gene on weight gain in morbid obesity" 1062-1066,

18 "Decuypere EA 2003 Cold-induced enhancement of avian uncoupling protein expression and triiodothyronine concentrations in broiler chicks" 70-77, 2003

19 "Clifton PM 2000 Association of -3826G variant in uncoupling protein-1 with increased BMI in overweight Australian women" 242-43 244, 2000

20 "Brand E 2003 Uncoupling protein 1 and 3 polymorphisms are associated with waist- to-hip ratio" 327-332, 2003

21 "Bouchard C 1994 DNA polymorphism in the uncoupling protein" 526-531,

22 "Apfelbaum M 1996 Polymorphisms of uncoupling protein and beta 3 adrenoreceptor genes in obese people submitted to a low calorie diet" 1051-1054, 1996