We investigated the characteristics and in vitro degradability of silk scaffold prepared by pressing method. SEM and u-CT results showed that the scaffold has some interconnected irregular pore. Human epithelial cell, HT-1376 was well attached and pro...
We investigated the characteristics and in vitro degradability of silk scaffold prepared by pressing method. SEM and u-CT results showed that the scaffold has some interconnected irregular pore. Human epithelial cell, HT-1376 was well attached and proliferated on the scaffold. XRD showed that the conformation of silk scaffold is mainly Silk II structure and some Silk I conformation. Degradability of silk scaffold was dependent on the density of silk materials in scaffold. Silk scaffolds were relatively stable in PBS solution (solution A) and cell culture medium (solution B). However, protease XIV degraded silk scaffold severely. From the above results, the degradability of silk scaffold could be controlled with the density of silk polymer. Silk scaffold might be applied for tissue engineering matrix.