선충 억제 활성 식물 추출물의 선발 = Screening of Plant Extracts for Nemastatic Activity

다국어 초록 (Multilingual Abstract)

A total of 2,714 methanol extracts of 1,677 plant species in 178 families were screened for nemastatic activityagainst Rhabditis sp. by 96-well microplate bioassay. The plant extracts with a concentration of 5,000 μg/mlwere mixed with aqueous nematode solution containing about 20 Rhabditis sp. and their activity was examineddaily for 7 days. Out of 2,714 plant extracts examined in this test, 2,362 (87.0%) showed no negative influenceon the nematode activity, while 187 (6.9%) inhibited nematode activity about 50%, 95 (3.5%) inhibitednematode activity over 90%, and 70 (2.6%) rather enhanced nematode activity. Among those showing over50% nemastatic activity, 25 extracts were randomly selected for further screening with Rhabditis sp. and withjuveniles of Meloidogyne incognita. The screening revealed that 11 extracts (44%) were consistently nemastatic.
The 282 extracts with nemastatic activity could be of use in future studies on nematicidal plants.

참고문헌 (Reference)

1 김성문, "고구마뿌리혹선충(Meloidogyne incognita)에 대한 국내 자생식물의 살선충 활성" 한국농약과학회 8 (8): 353-357, 2004

2 Miao, S. D, "Zhon ghua yao hai. (The expanse of Chinese herbal medicines)" Haerbin Publisher 1993

3 Prot., J. C, "Use of green manure crops in control of Hirschmanniella mucronata and H. oryzae in irrigated rice" 24 : 127-132, 1992

4 Verma, B. S, "Toxicity of some indigenous plant extracts to root-knot, seedgall, and citrus nematodes" 23 : 25-57, 1989

5 Siddiqui, M. A, "Toxicity of different plant parts of Tagetes lucida to plant-parasitic nematodes" 18 : 181-185, 1990

6 Whapham, C. A, "The role of seaweed extracts, Acophyllum nodosum, in the reduction in fecundity of Meloidogyne javanica" 17 : 181-183, 1994

7 Moriarty, Y, "The efficacy of chrysoidin, new blue R and phloxine B for determining the viability of beet eelwonn, Heterodera schachtii Schm" 10 : 644-646, 1964

8 Huang, K. C, "The Pharmacology of Chinese Herbs" CRC Press 388-, 1993

9 Mojtahedi, H, "Suppression of Meloidogyne chitwoodi with sudangrass cultivars as greenmanure" 25 : 303-311, 1993

10 Preiser, F. A, "Study and development of comparative laboratory testing of nematicides" McGill University 1962

11 Kim, D. G, "Simple cultivation of a Rhabditis sp. in nutrient broth" 39 : 42-46, 1997

12 MacGuidwin, A. E, "Response of nematode communities to sudangrass and sorghum-sudangrass hybrids grown as green manure crops" 27 : 609-616, 1995

13 Ferris, H, "Plant sources of Chinese herbal remedies" 31 : 241-263, 1999

14 Oostenbrink, M, "Nematology" Univ. of North Carolina 1960

15 Chatterjee, A, "Nematicidal principles from two species of Lamiaceae" 14 : 118-120, 1982

16 Grewal, P. S, "Nematicidal effects of some plant extracts to Aphelenchoides composticola (Nematoda) infesting mushroom, Agaricus bisporus" 12 : 317-322, 1989

17 Oka, Y, "Nematicidal activity of the leaf powder and extracts of Myrtus communis against the root-knot nematode Meloidogyne javanica" 61 : 1012-1020, 2012

18 Wiratno D. T, "Nematicidal activity of plant extracts against the root-knot nematode, Meloidogyne incognita" 2 : 77-85, 2009

19 Choi, I. H, "Nematicidal activity of medicinal plant extracts and two cinnamates isolated from Kaempferia galanga L. (Proh Hom) against the pine wood nematode, Bursaphelenchus xylophilus" 8 : 359-365, 2006

20 Satoshi T, "Nematicidal activity of Okinawa Island plants on the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood" 303 : 207-216, 2008

21 Perry, L. M, "Medicinal Plants of East and Southeast Asia" Massachusetts Institute of Technology 632-, 1980

22 Southey, J. F, "Laboratory Methods for Work with Plant and Soil Nematodes" Her Majesty’s Stationery Office 202-, 1986

23 McGaw, L. J, "In vitro anthelmintic, antibacterial and cytotoxic effects of extracts from plants used in South African ethnoveterinary medicine" 173 : 366-372, 2007

24 McGaw, L. J, "In vitro anthelmintic, antibacterial and cytotoxic effects of extracts from plants used in South African ethnoveterinary medicine" 173 : 366-372, 2007

25 Gamal Abdalla Elbadri, "Evaluation of various plant extracts for their nematicidal efficacies againstjuveniles of Meloidogyne incognita" 한국응용곤충학회 11 (11): 99-102, 2008

26 Tarjan, A. C, "Evaluation of various nematodes for use in contact nematicide tests" 22 : 33-37, 1955

27 Pavaraj, M, "Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne incognita" 5 : 106-110, 2012

28 Johnson, A. W, "Effects of rapeseed and vetch as green manure crops and fallow on nematodes and soil-borne pathogens" 24 : 117-126, 1992

29 Germani, G, "Effect of Sesbania rostrata on Hirschmanniella oryzae in flooded rice" 15 : 269-271, 1983

30 Heo, J, "Dongeuibogam" Yeogang Press 1994

31 Zhang, G, "Color Pictorial Handbook of Toxic Chinese Herbs" Xinhao Bookstore 1996

32 Taylor A. L, "Biology, Identification and Control of Root-knot Nematodes (Meloidogyne Species)" North Carolina State Univ 111-, 1978

33 Kim, H. J, "Biological control of northern root-knot nematodes, Meloidogyne hapla with plant extract" 37 : 199-206, 1998

34 Kuhajek, J. M, "A rapid microbioassay for discovery of novel fungicides for Phytophthora spp" 93 : 46-53, 2003