쥐의 대동맥손상 후 발생한 혈관내막증식증에서 Matrix Metalloproteinase(MMP) 발현의 의의 = The Significance of Expression of Matrix Metalloproteinases(MMPs) in Intimal Hyperplasia after Ballon Injured Rat Aorta

다국어 초록 (Multilingual Abstract)

Purpose: Vascular smooth muscle cells (VSMCs) migration and proliferation are important for neo-intimal formation after arterial injury. Migration of VSMCs requires degradation of basement membrane and extracelluar matrix surrounding the cell, and there is
increasing evidence that VSMCs produce extre-celluar matrix-degradating proteinases, such as matrix metalloproteinases (MMPs) after arterial injury.
To assess the role of MMPs in VSMCs
prolifetation, migration and intimal thickening, we measured the expression of MMP-2 and MMP-9 in the balloon-injured rat aorta model
Method: Twenty-five male Sprague-Dawley rats weighting of 250~300 gm were underwent aortic intimal depudation with 2F balloon catheter. Aorta was harvested at various time intervals of 1, 3, 5, 7, 21 days and then analyzed the MMP expression used by gelatin zymography. Intimal hyperplasia caused by balloon injury was
confirmed by microscopic examination.
Result: MMP-2 (72 kD) was constitutively
expressed in the normal aorta and was not increased substantially after injury. But eht expression of 62 kd forms, which is activated form of MMP-2, was significantly increased during the period of 5 through 7 days after injury (P < 0.05). The expression of MMP-9 (92 kd) was significantly increased at 1st day after injury and diminished thereafter (P < 0.05).
Conclusion: These results suggest that activated MMP-2 (62 kD) and MMP-9 (92 kD) may play an important role in VSMCs migration and formation of intitnal byper-plasia after arterial injury. And the activated form of MMP-2 (62 kD) seems to be involved mainly in degradation of basement membrane and matrix.